12. The K,m for the reaction of chymotrypsin with N-acetylvaline ethyl ester is 8.8x10-2 M, andthe K.," for the reaction of chymotrypsin with N-acetyltyrosine ethyl ester is 6.6x10 M.(a) Which substrate has the higher apparent affinity for the enzyme?(b) Which substrate is likely to give a higher value for Vmax?13. An enzyme catalyzes the reaction A B. The enzyme is present at a concentration of 2 nNMand the Vmax is 1.2 μΜ s-1. The Km for substrate A is 10 μΜ. Calculate the initial velocity of thereaction, Vo, when the substrate concentration is (a) 2 μΜ, (b) 10 μM, (c) 30 μΜ.14. An enzyme catalyzes the reaction M N. The enzyme is present at a concentration of 1 nM,and the Vmax is 2 μΜ s-1. The Km for substrate M is 4 μΜ. Calculate kcat.

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Asked Mar 28, 2019
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12. The K,m for the reaction of chymotrypsin with N-acetylvaline ethyl ester is 8.8x10-2 M, and
the K.," for the reaction of chymotrypsin with N-acetyltyrosine ethyl ester is 6.6x10 M.
(a) Which substrate has the higher apparent affinity for the enzyme?
(b) Which substrate is likely to give a higher value for Vmax?
13. An enzyme catalyzes the reaction A B. The enzyme is present at a concentration of 2 nNM
and the Vmax is 1.2 μΜ s-1. The Km for substrate A is 10 μΜ. Calculate the initial velocity of the
reaction, Vo, when the substrate concentration is (a) 2 μΜ, (b) 10 μM, (c) 30 μΜ.
14. An enzyme catalyzes the reaction M N. The enzyme is present at a concentration of 1 nM,
and the Vmax is 2 μΜ s-1. The Km for substrate M is 4 μΜ. Calculate kcat.
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12. The K,m for the reaction of chymotrypsin with N-acetylvaline ethyl ester is 8.8x10-2 M, and the K.," for the reaction of chymotrypsin with N-acetyltyrosine ethyl ester is 6.6x10 M. (a) Which substrate has the higher apparent affinity for the enzyme? (b) Which substrate is likely to give a higher value for Vmax? 13. An enzyme catalyzes the reaction A B. The enzyme is present at a concentration of 2 nNM and the Vmax is 1.2 μΜ s-1. The Km for substrate A is 10 μΜ. Calculate the initial velocity of the reaction, Vo, when the substrate concentration is (a) 2 μΜ, (b) 10 μM, (c) 30 μΜ. 14. An enzyme catalyzes the reaction M N. The enzyme is present at a concentration of 1 nM, and the Vmax is 2 μΜ s-1. The Km for substrate M is 4 μΜ. Calculate kcat.

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Expert Answer

Step 1

a) Km value indicates the substrate concentration at a rate of the reaction that is half the maximum value,i.e.,

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Step 2

Thus, for an enzyme with lower value of Km, large amount of substrate is required to achive the rate equal to half the maximum rate, which means poor affinity between the substrate and the enzyme.

Step 3

Thus, N-acetyltyrosineethyl ester has a higher affinity than N-ac...

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