13-2 For a and its regulatory sequences, the following mutation results have been found. gene Deletion Region % Transcription (lungs) % Transcription (kidneys) 100% Mutant # WT None 100% 1-200 250-400 130% 130% 100% 100% 500-800 50% 50% 100% 4 950-1100 0% Explain what type of sequence could have been mutated in each one.
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- 14) Do the cDNA libraries generated from a mouse brain and a mouse liver contain ALL of the same DNA sequences? Why or why not?Ch. 18-2 The photos shown below illustrate a case of synpolydactyly, a genetic abnormality characterized by two phenotypes: partially or completely duplicated fingers or toes, and webbing between fingers or toes. The same mutations that give rise to the human phenotype also give rise to a similar phenotype in mice. In which family of genes do you think these mutations occur?10. DNA clones, derived via reverse transcription of mRNA molecules into DNA and cloned into a vector form a ?
- 22-59 In the replication of a DNA molecule, two daughtermolecules, Q and R, are formed. The following basesequence is part of the newly formed strand in daughtermolecule Q.5′ ACTTAG 3′Indicate the corresponding base sequence ina. the newly formed strand in daughter molecule R.b. the “parent” strand in daughter molecule Q.c. the “parent” strand in daughter molecule R..14. DNA transcription may be selectively regulated through alteration of the rate of protein synthesis and differential degradation of mRNA true or falseQ10. Does frame +2 have an ORF in the coding region of this exon? What about frame +1 and frame +3? Q11. Given that 3 of the 64 possible codons are stop codons, what is the chance of having a stop codon at any given position, assuming that the sequence is random?
- Question:- 7. Describe how the Polycomb group proteins influence genome expression.BIOC 385 DNA Repair and Recombination Q10.2: The mRNA codon 5'-UGU-3' corresponds to 5'-TGT-3' in the DNA coding strand. Name each type of mutation - and the functional consequence - of the 3' thymidine deoxynucleotide being mutated to C, A, and G with regard to the encoded protein.Ch. 15-2 Restriction enzymes in bacterial cytoplasm cut injected bacteriophage DNA wherever certain sequences occur. A bacteria's chromosome also contains these sequences, and, being suspended in cytoplasm, it is also exposed to the enzymes. Why do you think the enzymes do not cut the chromosome?
- Q1: Why is only one strand of DNA used as a template? Q2: If a mutation occurred within the promoter or terminator region, do you think it would affect the mRNA transcribed? Why or why not? Q3: The template strand of part of a gene has the base sequence TGAGAAGACCAGGGTTGT. What is the sequence of RNA transcribed from this DNA, assuming that RNA polymerase travels from left to right on this strand?15. A method of sequencing long DNA fragments by generating new sequencing primers based on successive DNA reads is called?22-61 How does the synthesis of a daughter DNA strandgrowing toward a replication fork differ from the synthesis of a daughter DNA strand growing away from areplication fork?