15. Open end: You get the plasmid form your collaborator. The plasmid contains the gene of the protein for potential medical applications. In order to improve protein stability upon storage, you want to replace one amino acid, checking, of course, that it would not affect the activity of the protein. Also, the plasmid containing the gene is not an expression plasmid. Outline the steps of your experimental plan for this project with as many details as possible. Mention possible problem and the way you are going to overcome them.

Biology: The Dynamic Science (MindTap Course List)
4th Edition
ISBN:9781305389892
Author:Peter J. Russell, Paul E. Hertz, Beverly McMillan
Publisher:Peter J. Russell, Paul E. Hertz, Beverly McMillan
Chapter18: Dna Technologies: Making And Using Genetically Altered Organisms, And Other Applications
Section: Chapter Questions
Problem 3TYK: Why are antibiotic resistance markers such as ampR important components of bacterial plasmid cloning...
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15. Open end: You get the plasmid form your collaborator. The plasmid contains the gene of the protein
for potential medical applications. In order to improve protein stability upon storage, you want to
replace one amino acid, checking, of course, that it would not affect the activity of the protein. Also,
the plasmid containing the gene is not an expression plasmid. Outline the steps of your experimental
plan for this project with as many details as possible. Mention possible problem and the way you are
going to overcome them.
Transcribed Image Text:15. Open end: You get the plasmid form your collaborator. The plasmid contains the gene of the protein for potential medical applications. In order to improve protein stability upon storage, you want to replace one amino acid, checking, of course, that it would not affect the activity of the protein. Also, the plasmid containing the gene is not an expression plasmid. Outline the steps of your experimental plan for this project with as many details as possible. Mention possible problem and the way you are going to overcome them.
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