15. The CRISPR/Cas9 system provides a form of adaptive immunity in bacteria. Wediscussed in class that it can be used in human cells to:A: Protect cells from infection with virusesB: Kill cells that carry a specific deletionC: Give cells a memory of the viruses that have infected themD: Greatly enhance the frequency of homologous recombination at a chosenIlocusE: Kill cells that have not undergone the desired genome edit

The correct option is DThe CRISPR/Cas9 system is a bacterial immune system to provide protection…

Answered: 15. The CRISPR/Cas9 system provides a…

Biology: The Dynamic Science (MindTap Course List)

4th Edition

ISBN:9781305389892

Author:Peter J. Russell, Paul E. Hertz, Beverly McMillan

Publisher:Peter J. Russell, Paul E. Hertz, Beverly McMillan

Chapter17: Bacterial And Viral Genetics

Section: Chapter Questions

Problem 1TYK

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1. What makes CRISP particularly useful compared to other genetic engineering techniques? A. CRISPR can add DNA to multiple cells at the same time B. CRISPR works better in bacteria C. CRISPR can target one specific area of DNA to cut D. CRISP is capable of transporting DNA through the cell walls of plants 2. MOST viruses are capable of infecting A. many different species B. many cell types in the same species C. only specific cell types that have a particular receptor
Bacteria exposed to viruses incorporate sections of the virus’s DNA into the CRISPR array sequences in their genome. This mechanism allows bacteria to fight off the viruses, like an immune response: the information in CRISPR spacers served as “coordinates” for recognizing and cutting up invading DNA sequences. Describe what might happen under the conditions described after a bacteriophage infects a bacterial cell and releases its DNA into the bacterial cell. Explain why: 1. The invading phage DNA is recognized by the Cas proteins but not inserted into the CRISPR array region of the bacterial genome: The bacteria will be unable to elicit an immune response and will succumb to the phase infection 2. The cas genes on the bacterial genome contains a missense mutation that increases its cleavage/cut activityThe bacteria will elicit an immune response that will successfully fight the phage infection
5 How the use of the nucleic acid vaccine gives protection against diseases like COVID-19. Please briefly explain .?
Bacterial transformation is a major concern in many medical settings. Why might health care providers be concerned? Pathogenic bacteria could introduce disease-causing genes in non-pathogenic bacteria Antibiotic resistance genes could be introduced to new bacteria to create “superbugs. ” Bacteriophages could spread DNA encoding toxins to new bacteria All of the above.
Which of the following is not correct for bacterial conjugation? a. Both Hfr and F+ bacteria have the ability to code for a sex pilus. b. After an F- cell has conjugated with an F+, its plasmid holds the F+ factor. c. The recipient cell is Hfr following conjugation. d. In an HfrF- conjugation, DNA of the main chromosomemoves to a recipient cell. e. Genes on the F factor encode proteins of the sex pilus.
Which of the following sequences on a DNA molecule would be complementary to GCTTATAT? TAGGCGCG ATCCGCGC CGAATATA TGCCTCTC
Why are antibiotic resistance markers such as ampR important components of bacterial plasmid cloning vectors? a. The plasmid must have resistance to accept DNA inserts. b. They allow the detection of plasmids that contain an inserted DNA fragment. c. They ensure the presence of the ori site. d. They ensure that the plasmid can be cut by a restriction enzyme. e. They allow identification of bacteria that have taken up a plasmid.
What is the most challenging issue facing genome sequencing? the inability to develop fast and accurate sequencing techniques the ethics of using information from genomes at the individual level the availability and stability of DNA all of the above
The chain termination method of sequencing uses labeled ddNITPs uses only dideoxynucleotides uses only deoxynucleotides uses labeled dNTPs
1. An alternative form of a gene is called an
1. How may recombinant DNA molecules be introduced into human cells? a. by splicing the needed genes into a mammalian chromosome using restriction enzymes. b. by adding plasmids to the mammalian cells. c. by using engineered viruses as vectors. d. by using a gene gun. 2. If someone is accused of a crime, investigators can collect his or her DNA to compare the DNA of the cells found at the crime scene. To collect human DNA, investigators often swab the inside of person’s cheek. Just a few human cheek cells contain enough material to perform PCR. In a cell, the nucleus and mitochondria contain DNA that is the starting material for PCR. Identify the 4 components needed to start a PCR reaction (equipment not included)
1. What is the function of the CRISPR/ Cas system 2. Briefly outline the components of the CRISPR/Cas system

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