Question
16. Why is re-circularized insert generally not a problem in cloning procedures, while re-
circularized vector can be a problem? Is it because re-circularized insert:
A: is detrimental to bacteria
B: is a kind of product that DNA ligase cannot form
C: makes E. coli sensitive to antibiotics
D: lacks a replication origin
E: is beneficial to bacteria
om
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Transcribed Image Text:16. Why is re-circularized insert generally not a problem in cloning procedures, while re- circularized vector can be a problem? Is it because re-circularized insert: A: is detrimental to bacteria B: is a kind of product that DNA ligase cannot form C: makes E. coli sensitive to antibiotics D: lacks a replication origin E: is beneficial to bacteria om
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