2. Balqees was all ready to observe the 3 slides but when she looked into the microscope she was surprised to see that only half of the view/ field is illuminated the other half is dark. What could be the probable cause? 3. After fixing the illumination issue Balqees thought of looking at the amoeba slide but she realized that she couldn't see the amoeba in the slide. List 3 possible mistake she is making?

A student missed the laboratory period where the use of the microscope was demonstrated. The instructor asked the student to read the description in the laboratory manual and then proceed to examine bacterial cells with the oil immersion lens. The student skimmed the directions and began. After about 15 minutes of struggling, the student gave up in despair without seeing anything. Below is a detailed description of what the student did. How many mistakes did the student make and why didn’t the student see anything? a. Plugged in the microscope and turned the light source to maximum intensity. Made a wet mount and placed it on the stage with the low-power objective lens in position. Tried to focus with the coarse adjustment, but decided the bacteria were too small and needed to be seen with the high-power objective lens. Rotated the high-power objective lens into position, but saw the lens would likely touch the slide, so lowered the stage so that the objective lens rotated freely.…

A- This is a microscopy image of a club moss (Lycopodiaceae) strobilus. B- In this image, the strobilus carries different types of spores. 500 µm

12. A student makes a smear by applying a bacteria onto the slide. A simple stain is performed, but when the student looks under the microscope, no organisms are seen. What did the student do wrong when making the smear?

1. Why is it helpful that the transmission compound light microscopes we studied in lab are both parfocal and parcentered? O Why would it be harder to find a specimen on a slide starting with the 40x objective lens compared to the 4x objective lens? Support your answer with facts about the field of view and the comparison of both objective lenses. If I wanted to observe something, let's a virus that was 50 nm in size which microscope would you recommend me using? Support your answer with facts about the microscope's utility.

Image A: Image B: 25 um O A. image A- scanning electron microscope, Image B- phase contrast microscope O B. Image A brightfield microscope; Image B- darkfield microscope O C.image A- brightfield scanning microscope Image B- phase contrast scanning microscope D. image A- transmission electron microscope, Image B- scanning electron microscope O E. image A- scanning electron microscrope, Image B- transmission electron microscope

question 6