2. You use tubes to test aerotolerance of bacteria. From your samples you have 3 results: A.Bacteria growing on the surface. B. Bacteria growing throughout the tube, the agar shows cracks.C. Bacteria growing about 5 mm below the surface. Please interpret each bacterial result. (Give thebacteria an oxygen classification, explain what classification means and interpret the cracks in theagar.)3. Please explain how the use of an Eosin Methylene Blue Agar plate can help determine the typeof fermenters that bacteria are. Please explain thoroughly.

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Asked Feb 28, 2020
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2. You use tubes to test aerotolerance of bacteria. From your samples you have 3 results: A.
Bacteria growing on the surface. B. Bacteria growing throughout the tube, the agar shows cracks.
C. Bacteria growing about 5 mm below the surface. Please interpret each bacterial result. (Give the
bacteria an oxygen classification, explain what classification means and interpret the cracks in the
agar.)
3. Please explain how the use of an Eosin Methylene Blue Agar plate can help determine the type
of fermenters that bacteria are. Please explain thoroughly.
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2. You use tubes to test aerotolerance of bacteria. From your samples you have 3 results: A. Bacteria growing on the surface. B. Bacteria growing throughout the tube, the agar shows cracks. C. Bacteria growing about 5 mm below the surface. Please interpret each bacterial result. (Give the bacteria an oxygen classification, explain what classification means and interpret the cracks in the agar.) 3. Please explain how the use of an Eosin Methylene Blue Agar plate can help determine the type of fermenters that bacteria are. Please explain thoroughly.

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Expert Answer

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Hello, thank you for your question. As per our policy, I am going to answer only the first question. If you need help regarding the second question please post it separately.

Step 2

Aerotolerance test is performed on the microbes to determine whether it is aerobic or anaerobic. 

Anaerobic bacteria or aerotolerant organisms can be identified by growing them in test tubes of thioglycollate broth.

The types of bacteria you can observe on performing aerotolerance test:
1: Obligate aerobes- need oxygen because they cannot ferment or respire anaerobically. They gather at the top of the tube where the oxygen concentration is highest.
2: Obligate anaerobes- are poisoned by oxygen, so they gather at the bottom of the tube where the oxygen concentration is lowest.
3: Facultative anaerobes- can grow with or without oxygen because they can metabolize energy aerobically or anaerobically. 
4: Microaerophiles- need ox...

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