2. You use tubes to test aerotolerance of bacteria. From your samples you have 3 results: A. Bacteria growing on the surface. B. Bacteria growing throughout the tube, the agar shows cracks. C. Bacteria growing about 5 mm below the surface. Please interpret each bacterial result. (Give the bacteria an oxygen classification, explain what classification means and interpret the cracks in the agar.) 3. Please explain how the use of an Eosin Methylene Blue Agar plate can help determine the type of fermenters that bacteria are. Please explain thoroughly.
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- 1. How is UV radiation a good type of control mechanism against microbial growth? Please explain what happens to the microbe and effects this control causes. 2. Suppose you do the Kirby-Bauer test on a hypothetical Staphylococcus species with penicillin and tetracycline. You record diameters of 20mm for tetracycline and 24mm for penicillin. Which antibiotic is most effective against this bacterium and why? Please explain and interpret these results.2. Suppose you do the Kirby-Bauer test on a hypothetical Staphylococcus species with penicillin and tetracycline. You record diameters of 20mm for tetracycline and 24mm for penicillin. Which antibiotic is most effective against this bacterium and why? Please explain and interpret these results.2. A mannitol-salt agar plate was inoculated with these bacteria and is shown below. A. What type of organisms grow on this medium? B. Based on the reaction below, what can you say about the organism derived from the patient's abscess?
- 1. Why do you think the 5% sheep blood agar was selected for the culture of mouth bacteria?1) would you describe the contents of the soil-inoculated broth as being a “pure culture”? Why or why not? 2) How did the uninoculated broth differ in appearance from the broths inoculated with E. Coli and M. Luteus? And then how could you tell if a supposedly sterile, uninoculated broth was contaminated? Please explain in detail and highlight the important parts cuz I am confused and need help! Thanks2. You use tubes to test aerotolerance of bacteria. From your samples you have 3 results: A. Bacteria growing on the surface. B. Bacteria growing throughout the tube, the agar shows cracks. C. Bacteria growing about 5 mm below the surface. Please interpret each bacterial result. (Give the bacteria an oxygen classification, explain what classification means and interpret the cracks in the agar.)
- 22) Which of the techniques/characteristics below may be used to identify a target bacterium in a pool of microorganism? a) its morphology b) its growth requirement c) antibody-antigen interaction ) d) all of the above. ) e) a and c 23) What is the process that produces alcohol in S. cerevisiae (yeast)? () a) Respiration ( b) Sedimentation c) Photosynthesis )d) Fermentation 24) Identify the most mistaken (wrong) choice: a) Halophiles are those organisms that like high salt concentrations. ) b) Some bacteria reproduce by binary fission. ( c) A fungus cell contains a true nucleus. d) During the lag phase of growth, the metabolic activity is very low11. A selective media a) inhibit the growth of certain bacteria while allowing others to grow b) allow for visual distinction of the ability to perform a specific biochemical process c) Will only grow Gram-positive bacteria d) allow just the bacteria to grow1. A plate with a final dilution factor of 107 produced 210 colonies. a) What was the original concentration in the sample? b) If you were to dilute the original sample with a dilution factor of 108, how many colonies would you count on the plate? c) If you were to dilute the original sample with a dilution factor of 106, how many colonies would you count on the plate? 2. Sally Monella found 344 bacterial colonies on one of her plates. She had prepared this plate after a serial dilution of a culture of Yersinia pestis. She had pipetted 1 ml sample of diluted Yersinia pestis from a tube with the overall dilution factor of 106 into a plate and covered it with agar. She used this plate to calculate the concentration in cells/ml of a bacterial culture. a) How many organisms were in the original culture? b) Were her results valid? Why or why not?
- 1. In Microbiology, are there technologies that can help make bacterial culture and sensitivity done faster?1 (a)Why is the spectrophotometer set at 0.000 absorbance for the uninoculated tube of broth? (B) Why can't you use the same plot (or standard curve) of plot of absorbance versus cell count for Escherichia coli for other bacteria? (C)List 3 advantages of estimating microbial numbers or biomass by the turbidimetric method? (C)(ii)List 2 disadvantages of this method? (D) Can you measure all kinds of microbes this way? Why not? ( this is not a graded assignment)1. You were given a mixed nutrient agar broth culture of bacteria. a. How will you determine the different types of bacteria present in the mixed culture? b. How will you make a pure culture of these bacteria in a slant nutrient agar? c. How will you identify these bacteria?