2:16 Bacterial Growth Lab Back General Microbiology_23210 Use this data: time 30 oC 37 oC Minutes) OD 550 nm OD 550 nm 0.05 0.05 20 0.06 0.07 40 0.1 0.21 60 0.16 0.44 80 0.28 0.95 Graph your data on semi-log paper so that you get a straight line during the log phase. semi log_numbered[1]_(2).pdf As an alternative, I highly recommend using a spreadsheet- Excel, Google sheets-to plot the data. I will work on a tutorial for this. 4- Record your data in your lab notebook. Keep the graphs handy. Dashboard Calendar To Do Notifications Inbox
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- 22) Which of the techniques/characteristics below may be used to identify a target bacterium in a pool of microorganism? a) its morphology b) its growth requirement c) antibody-antigen interaction ) d) all of the above. ) e) a and c 23) What is the process that produces alcohol in S. cerevisiae (yeast)? () a) Respiration ( b) Sedimentation c) Photosynthesis )d) Fermentation 24) Identify the most mistaken (wrong) choice: a) Halophiles are those organisms that like high salt concentrations. ) b) Some bacteria reproduce by binary fission. ( c) A fungus cell contains a true nucleus. d) During the lag phase of growth, the metabolic activity is very low12/ Which of the following factors increases biofilm accumulation? (select all that apply) V A. malocclusion V B. smooth surfaces V C. calculus V D. restorations E. use of fluoridated 9/ During biofilm formation, microcolonies form in layers as the bacterial colonies multiply. With increased size, colonies meet and coalesce to form a complex microbial community. © A Both statements are true. A © B Both statements are false. C. The first statement is true, and the second statement is false. D. The first statement is false, and the second statement is true. Question 11 Which of the following is found in a disease periodontal pocket? © A Gram positive cocci and lactobacillus species. O B. Primarily jam negative motile rods and spirochetes • C. Gram positive aerobes © D. Keystone pathogens.In a laboratory setting, E. coli has a doubling time of 20 minutes.a) What is its growth rate, r?b) Assuming you start with only 2 cells, what will be the bacterial population after 24hourshave passed?
- If the temperature of the incubator were to be increased from 35 to 45°C, how would this affect the bacterial growth curve of E. coli? (optimum temperature 20o-40oC; most human pathogens)12. A differential media a) allows visual distinction between bacteria to perform a specific biochemical process b) inhibits the growth of certain bacteria c) will only grow Gram-negative bacteria d) is a selective mediaA sample of raw ground beef initially contained 25 E. Coli bacteria. After six hours, there are 102 400 cells in the sample. What is the growth rate of the E. coli population? Show all work. Express your answer using scientific notation in the form of xyz 10 d #bacteria/hr.
- 1. What is the appropriate and economically wise preservation method for a thermophilic endospore forming bacterium? Discuss and explain it comprehensively, and please, do not just copy from somewhere. Choose among this methods: periodic transfer to fresh media, overlaying cultures with mineral oil, freeze-drying, cryopreservation, drying, sterile distilled waterIf a culture of E. coli you are tracking grows from 5 x 103 cells/ml to 9 x 107 cells per ml in 4 hours, what is the approximate generation time of your E. coli? how can i slove this step by step a. About 14 minutesb. About 17 minutesc. About 20 minutesd. About 23 minutese. About 30 minutesMicrobiology BIOL 2420 Question 10 In an optional exercise that we did not have time to do, nutrient agar plates were inoculated with samples taken from the zones of inhibition. If there was growth on the NA plate after incubation, what would that tell you about the antibiotic? Question 10 options: a) that antibiotic is bacteriostatic b) that antibiotic is bactericidal c) that the therapeutic index was high d) that the dosage was smaller than the minimum inhibitory concentration
- 47. All the following characteristics can describe bacterial colony’s shape and form except: a) Colony margins b) Form c) surface characteristics d) presence or absence 0f an outer membrane in the bacteria 48. A box shaped bacterial arrangement made of 8 spherical looking bacteria is called: a) Staphylobacillus b) Sarcina c) Octococcus d) Diplotetrad 49.22) Which of the techniques/characteristics below may be used to identify a target bacterium in a pool of microorganism? ( a) its morphology () b) its growth requirement c) antibody-antigen interaction d) all of the above. ( e) a and c 23) What is the process that produces alcohol in S. cerevisiae (yeast)? ( a Respiration ( b) Sedimentation c) Photosynthesis ) d) FermentationHow does the streak plate technique help in isolating individual colonies of bacteria? Why streak multiple times on one plate? 3. While observing a plate inoculated with bacteria you observe a white-tan fuzzy growth. What can you interpret from this observation? Explain how this outcome could’ve occurred. 4. What are 3 possible errors that could take place as you culture your samples which could lead to contamination? State 3 and explain why. Microbiology class