3’ G G A U A C G U C A C C G G U A U A A G G U U U C G U A U C G 5’ If the RNA synthesized above is a functional mRNA and all the nucleotides belong to an exon, a. how many codons are present in this mRNA?                          b. how many codons actually code for a protein in this mRNA? c. what stop codon is present in this mRNA?

4a) Write out the protein sequence (the amino acids, in order) encoded by the mRNA sequence:               5'AUGCGACCUAGCUAUGGA3'

First Position (5'-end) U C A U UUU Phe UUC Phe UUA Leu UUG Leu G CUU Leu CUC Leu CUA Leu CUG Leu AUU Ile AUC Ile AUA Ile AUG* Met GUU Val GUC Val GUA Val GUG Val с UCU UCC UCA UCG Ser Second Position A CCU Pro CCC Pro CCA Pro CCG Pro ACU ACC Ser Ser Ser Thr Thr ACA Thr ACG Thr GCU GCC Ala Ala GCA Ala GCG Ala *AUG also serves as the principal initiation codon. G UAU Tyr UGU UAC Tyr UGC UAA Stop UGA UAG Stop UGG CAU His CAC His CAA Gln CAG Gln AAU Asn AAC Asn AAA AAG GAU Asp GAC Asp GAA Glu GAG Glu CGU Arg CGC Arg CGA Arg CGG Arg Lys AGA Lys AGG Cys Cys Stop Trp AGU Ser AGC Ser GGU GGC Arg Arg Gly Gly GGA Gly GGG Gly Third Position (3'-end) DOAG DOAG U C U C DOAG DOAG U C U C

1c) Give the anticodon of the tRNA that would be a "wobble match" for the codon 5'AGG3'.