3. Approximate molecular weight for an unknown protein from gel-filtration experiment is 130 kDa. Thirty six mg of this pure protein was treated with excess of fluorodinitrobenzene. After the reaction and complete acid hydrolysis the mixture was found to contain 356 µg of dinitrobenzene derivative of methionine (free acid) and no other amino acid-dinitrobenzene derivatives. Is this protein consisting of single polypeptide chain or multiple subunits? If it consists of multiple subunits, then how many? From these data, can you calculate the molecular weight of the protein more precisely?

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3. Approximate molecular weight for an unknown protein from gel-filtration experiment is 130
kDa. Thirty six mg of this pure protein was treated with excess of fluorodinitrobenzene. After the
reaction and complete acid hydrolysis the mixture was found to contain 356 µg of dinitrobenzene
derivative of methionine (free acid) and no other amino acid-dinitrobenzene derivatives. Is this
protein consisting of single polypeptide chain or multiple subunits? If it consists of multiple
subunits, then how many? From these data, can you calculate the molecular weight of the protein
more precisely?
Transcribed Image Text:3. Approximate molecular weight for an unknown protein from gel-filtration experiment is 130 kDa. Thirty six mg of this pure protein was treated with excess of fluorodinitrobenzene. After the reaction and complete acid hydrolysis the mixture was found to contain 356 µg of dinitrobenzene derivative of methionine (free acid) and no other amino acid-dinitrobenzene derivatives. Is this protein consisting of single polypeptide chain or multiple subunits? If it consists of multiple subunits, then how many? From these data, can you calculate the molecular weight of the protein more precisely?
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