3. How many milliliters would you need to prepare a 10-2 dilution from a 10ml starting culture?

Time point (min) Absorbance of culture at 660nm Approximate cell concentration Approximate # cells in 1mL extract 0 0.298 1.49 x 108 cells/mL 1.49 x 108 cells 10 0.316 1.58 x 108 cells/mL 1.58 x 108 cells 20 0.374 1.87 x 108 cells/mL 1.87 x 108 cells 30 0.429 2.145 x 108 cells/mL 2.145 x 108 cells 40 0.512 2.56 x 108 cells/mL 2.56 x 108 cells 50 0.544 2.72 x 108 cells/mL 2.72 x 108 cells 60 0.607 3.035 x 108 cells/mL 3.035 x 108 cells a. Using these data, prepare a growth curve of this strain ofEscherichia coli (E. coli).b. Estimate the doubling time for this strain of E. Coli. Clearly showhow you estimated this value from the empirical data presented.

The following image is of an agarose gel. If DNA samples were loaded to this gel and the electrophoresis experiment was started, explain what would happen and why.

Gradient PAGE uses differing gel concentrations along the length of the gel to achieve optimalseparation of proteins of a wide range of molecular weight proteins. How does the staining pattern of these types of gels differ from nongradient PAGE?

20. According to the package insert, adding 2.5 mL of sterile water to a 1 g cefazolin vial yields a final volume of 3 mL. What volume of this solution would you need to obtain 200 mg of cefazolin?

d/e/1FAIpQLSfTle9UfP15_VUqFI-ACEQd1XBykXv5Lr4dEMQbLJ1d6fCupw/viewform Students subjected three samples of five different molecules to gel electrophoresis as shown in Figure 1 A B C DE +2 3 Wells 4 8. Which of the following statements best explains the pattern seen on the * gel with regard to the size and charge of molecules A and B? 1 point molecules A and B are positively charged, and molecule A is smaller than molecule B. molecules A and B are positively charged, and molecule A is larger than molecule B. molecules A and B are negatively charged, and molecule A is smaller than molecule B. molecules A and B are negatively charged, and molecule A is larger than molecule B. Sign out

The DNA fragments separated on an agarose gel can be visualised after staining with what?

Besides your mycobacteria, what other microorganism can appear as acid fast positive when processed using acid fast staining?

Outline the techniques of two-dimensional gel electrophoresisand tandem mass spectroscopy, and explain why they are used.

Oops!  Your pipettor wasn’t set correctly and you only pipetted 87 µl of a 10-7 dilution when plating serial dilutions.  But, you noted this error in your lab notebook so you could correctly perform the calculation.  When this plate was grown, you counted 147 colonies on the plate.  What was the concentration of bacteria in the original, undiluted suspension?