3. Now that the sequence of the entire E. coli K12 straingenome (roughly 5 Mb) is known, you can determineexactly where a cloned fragment of DNA came fromin the genome by sequencing a few bases and matching that data with genomic information.a. About how many nucleotides of sequence information would you need to determine exactly where afragment is from?b. If you had purified a protein from E. coli cells,roughly how many amino acids of that proteinwould you need to know to establish which geneencoded the protein?c. You determine 100 nucleotides of sequence ofgenomic DNA from a different E. coli strain, butyou cannot find a match in the E. coli K12 genomesequence. How is this possible?
3. Now that the sequence of the entire E. coli K12 strain
genome (roughly 5 Mb) is known, you can determine
exactly where a cloned fragment of DNA came from
in the genome by sequencing a few bases and matching that data with genomic information.
a. About how many
fragment is from?
b. If you had purified a protein from E. coli cells,
roughly how many amino acids of that protein
would you need to know to establish which gene
encoded the protein?
c. You determine 100 nucleotides of sequence of
genomic DNA from a different E. coli strain, but
you cannot find a match in the E. coli K12 genome
sequence. How is this possible?
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