3. Two mobile phases were evaluated to study the behavior of five dyes. The followingdata from each chromatogram were collected:Mobile Phase 1: Acetone/WaterRFMobile Phase 2: Ethanol/WaterDistancetraveled (cm)RFDistancetraveled (cm)4.74.9Solvent front0.620,ая0.850.620.660.980.760.160.530.294.82.9Green dyeRed dyeYellow dyeBlue dyePurple dye3.71.30.84.02.62.91.43.1a) Calculate the RF values for each dye for both chromatographic systems. Record thesevalues in the table above.b) Explain your reasons for choosing which solvent would be most effective inseparating the green from the blue dye.c) Which mobile phase system would be most effective for separating all 5 dyes? Explain.

(a) Retention factor (Rf) is the ratio of the distance moved by the solute (dye) to the distance…

Answered: 3. Two mobile phases were evaluated to…

Fundamentals Of Analytical Chemistry

9th Edition

ISBN:9781285640686

Author:Skoog

Publisher:Skoog

Chapter33: High-performance Liquid Chromatography

Section: Chapter Questions

Problem 33.14QAP

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