Homework Help is Here – Start Your Trial Now!
Science
Biochemistry
3a)ClustalX software was used to perform multiple sequence alignment of the following five Nco protein sequences designated as Nco1-Nco5 (the provided figure). A pair of degenerate primers was designed to PCR-amplify a DNA segment with the size of approximately 290 bp. With justification, discuss which amino acid sequence blocks would be suitable to design the forward and reverse degenerate primers.

3a)ClustalX software was used to perform multiple sequence alignment of the following five Nco protein sequences designated as Nco1-Nco5 (the provided figure). A pair of degenerate primers was designed to PCR-amplify a DNA segment with the size of approximately 290 bp. With justification, discuss which amino acid sequence blocks would be suitable to design the forward and reverse degenerate primers.

Case Studies In Health Information Management
Case Studies In Health Information Management
3rd Edition
ISBN:9781337676908
Author:SCHNERING
Publisher:SCHNERING
Chapter6: Leadership
Section: Chapter Questions
Problem 6.33.3C
See similar textbooks
icon
Related questions
Question

3a)ClustalX software was used to perform multiple sequence alignment of the
following five Nco protein sequences designated as Nco1-Nco5 (the provided figure). A pair of degenerate primers was designed to PCR-amplify a DNA segment with the size of approximately 290 bp. With justification, discuss which amino acid
sequence blocks would be suitable to design the forward and reverse degenerate primers.

Nco2 Nco3 Nco4 Ncol Nco5 VWFCDPKWDS)IRLILKAKAONTVMDLVRELGTVEDLELEDVMKVGYGGVKCVESGGPGPG VWFCDPKWDSVRLILKAKAQATVMDLVRERGTVEDLELDDVLKIGYGNAKCVESGGPEPG AWFCDPKWDAVRLILKRKAONTVMDMAREKGTVEDLEIDEVLLHGFKGVKCAESGGPWPG VWFCDPKWDSVKLIMKGKKOPSVLDTLRKEGSSDDKELEDVMKIGFGGIRCVESGGPSPG TWFCDPKWDSTRLILKGLAQKTVLDTLREEGEWWDVDLEDIRREGFGECLCTESGGPAPG **************** ******* ******** Nco2 Nco3 Nco4 Ncol Nco5 VICAGRGVITAINFLEENGAYTDGSTLDFVYQWSRYEWVLDGIAMPIREGKFSPTALRGL WCAGRGVITAINFLEENGAYTPDGALDFVYQWSRYEWVIPWDASPIRENKFSPTALRGL WCAGRGVITAINFLECNGAYNEDKRTDFVYQWSRYEWIIESEAHPIREGKYSPTALRGL WCAGRGIITSIGLLENLGAYTDDGALDYVYOWSRYEWVLDEPAMPIREGKFSPTALRGL VICAGRGIITSINLLEQLGAYNEDQKLDYIYOWSRYEWILEGDAKPIREGKYSPTALRGL %3D ***** *********
Transcribed Image Text:Nco2 Nco3 Nco4 Ncol Nco5 VWFCDPKWDS)IRLILKAKAONTVMDLVRELGTVEDLELEDVMKVGYGGVKCVESGGPGPG VWFCDPKWDSVRLILKAKAQATVMDLVRERGTVEDLELDDVLKIGYGNAKCVESGGPEPG AWFCDPKWDAVRLILKRKAONTVMDMAREKGTVEDLEIDEVLLHGFKGVKCAESGGPWPG VWFCDPKWDSVKLIMKGKKOPSVLDTLRKEGSSDDKELEDVMKIGFGGIRCVESGGPSPG TWFCDPKWDSTRLILKGLAQKTVLDTLREEGEWWDVDLEDIRREGFGECLCTESGGPAPG **************** ******* ******** Nco2 Nco3 Nco4 Ncol Nco5 VICAGRGVITAINFLEENGAYTDGSTLDFVYQWSRYEWVLDGIAMPIREGKFSPTALRGL WCAGRGVITAINFLEENGAYTPDGALDFVYQWSRYEWVIPWDASPIRENKFSPTALRGL WCAGRGVITAINFLECNGAYNEDKRTDFVYQWSRYEWIIESEAHPIREGKYSPTALRGL WCAGRGIITSIGLLENLGAYTDDGALDYVYOWSRYEWVLDEPAMPIREGKFSPTALRGL VICAGRGIITSINLLEQLGAYNEDQKLDYIYOWSRYEWILEGDAKPIREGKYSPTALRGL %3D ***** *********
Expert Solution
steps

Step by step

Solved in 2 steps with 2 images

SEE SOLUTIONCheck out a sample Q&A here
Blurred answer
Recommended textbooks for you
Case Studies In Health Information Management
Case Studies In Health Information Management
Biology
ISBN:
9781337676908
Author:
SCHNERING
Publisher:
Cengage
Essentials of Pharmacology for Health Professions
Essentials of Pharmacology for Health Professions
Nursing
ISBN:
9781305441620
Author:
WOODROW
Publisher:
Cengage
Intro To Health Care
Intro To Health Care
Health & Nutrition
ISBN:
9781337338295
Author:
Mitchell
Publisher:
Cengage
SEE MORE TEXTBOOKS