4. You plate 3 different strains of the bacteria family Aspergillis on a MacConkey Agar plate. Younotice that 2 have a yellow shade, 1 has a subtle pink color. Please interpret this information. Suchas, the type of fermenters these bacteria are and what this says about their metabolism. What doesthe color change represent? And how is this color change possible?5. You observe a blood agar plate and see a clear/transparent area where the bacteria has beeninoculated. With this information what are some possible outcomes if someone was infected withthis type of bacteria? Please explain.

Bacterial culture media are the artificial medium that provides natural-like conditions to bacteria…

Answered: 4. You plate 3 different strains of the…

Biology: The Dynamic Science (MindTap Course List)

4th Edition

ISBN:9781305389892

Author:Peter J. Russell, Paul E. Hertz, Beverly McMillan

Publisher:Peter J. Russell, Paul E. Hertz, Beverly McMillan

Chapter26: Prokaryotes: Bacteria And Archaea

Section: Chapter Questions

Problem 8TYK

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2. You use tubes to test aerotolerance of bacteria. From your samples you have 3 results: A. Bacteria growing on the surface. B. Bacteria growing throughout the tube, the agar shows cracks. C. Bacteria growing about 5 mm below the surface. Please interpret each bacterial result. (Give the bacteria an oxygen classification, explain what classification means and interpret the cracks in the agar.)
1. On your groupmate's DCC plate, you can see mucoid bacterial colonies with clearings underneath. What all can they conclude based only on the DCC test? Select all that apply. a. The bacteria ferments glucose. b. The bacteria produces acid byproducts from fermentation. c. The bacteria can aerobically respire. d. The bacteria does not produce gas byproducts..
1. How is UV radiation a good type of control mechanism against microbial growth? Please explain what happens to the microbe and effects this control causes. 2. Suppose you do the Kirby-Bauer test on a hypothetical Staphylococcus species with penicillin and tetracycline. You record diameters of 20mm for tetracycline and 24mm for penicillin. Which antibiotic is most effective against this bacterium and why? Please explain and interpret these results. 3. Please provide the scientific name of your microbe that was used in the UV experiment (i.e. S. aureus). Compare your plates and interpret/analyze your results. Please discuss your findings and any patterns you were able to gather. 4. After performing the “Effects of Antiseptics & Disinfectants” lab which agent(s) showed potential to control S. marcescens growth? P. aeruginosa? Please explain why you believe these agent(s) work. 5. What purpose does water serve in the “Effects of Antiseptics & Disinfectants” lab? What did you…
1. Why is it important to have isolated bacterial colonies for this lab activity (Bacteria of GI Tract)? What results would you obtain if you had a mixed culture of bacteria?
1. How is UV radiation a good type of control mechanism against microbial growth? Please explain what happens to the microbe and effects this control causes. 2. Suppose you do the Kirby-Bauer test on a hypothetical Staphylococcus species with penicillin and tetracycline. You record diameters of 20mm for tetracycline and 24mm for penicillin. Which antibiotic is most effective against this bacterium and why? Please explain and interpret these results.
You plate 3 different strains of the bacteria family Aspergillis on a MacConkey Agar plate. You notice that 2 have a yellow shade, 1 has a subtle pink color. Please interpret this information. Such as, the type of fermenters these bacteria are and what this says about their metabolism. What does the color change represent? And how is this color change possible?
1. What is the purpose of an autoclave and what is its mechanism?2. Why is there a need for an incubator in the lab? How about incubating cultures at roomtemperature? How can this affect microbes?3. What are the different hoods in the lab and why are they important?4. How does the light compound microscope magnify an object? Outline the process5. Explain why an inverted image is seen under a compound microscope.6. What is the purpose of the oil when using oil immersion objective?7. Why should the microscope be calibrated for each objective and prior to each use?8. When carrying the microscope, why should one hand hold the bottom of the base?
1 (a)Why is the spectrophotometer set at 0.000 absorbance for the uninoculated tube of broth? (B) Why can't you use the same plot (or standard curve) of plot of absorbance versus cell count for Escherichia coli for other bacteria? (C)List 3 advantages of estimating microbial numbers or biomass by the turbidimetric method? (C)(ii)List 2 disadvantages of this method? (D) Can you measure all kinds of microbes this way? Why not? ( this is not a graded assignment)
1. If the yogurt were contaminated, how would thr gram stain look like? And how will it affect the taste and smell of the yogurt? 2. How are microbial fermentations used to preserve food?
4) You are interested in the total bacterial load of the bat guano. In order to determine this, you go back to your original broth culture and prepare a dilution series. You then spread 100 ml of each dilution onto 3 separate plates. You obtain the following results: Plate 1, 10 -1 dilution: 362 colonies Plate 2, 10 -3 dilution: 76 colonies Plate 3, 10 -5 dilution: 8 colonies. Based on your results, calculate the CFUs/ml in your original broth culture. show your work
8. Some antibiotics show no zone of inhibition against the microbe. How is this possible? Explain how the microbe is able to combat against the antibiotic with proper reasoning.
10. The use of the autoclave ensures: a) the death of all bacteria b) the death of all bacterial spores c) all of the above d) none of the above.11. Foods can be preserved in all the followings except: a) lyophilizing b) Drying c) Salting d) Fermentation12. The temperature of the autoclave should be held for _____ minutes at least: a) 10 b) 15 c) 20 d) 30

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