50 g of food is homogenized in 450 mL of water. From this, 1 mL is added to 29 mL of water, and then a 1:90 dilution is made from this, and then 1 mL from this is added to 99 mL of water, and then 100 uL of this last dilution is plated and after incubation 164 colonies are counted.a) What was the overall dilution factor?b) What was the total CFU/g of the original sample?

Why are only few drops of CuSO4 solution added during the biuret test?

How do you calculate the initial concentration of a sample? How would you make a 1:10 dilution of a broth culture if the final volume of the dilution is 10 ml? How much broth, and how much diluent, would you add? If you made a series of tenfold dilutions, exactly the same way as you described in question 1, what would be the dilution factor in the fifth tube? The following are errors that people commonly make when they perform serial dilutions. Indicate whether you think that the number of cfu/ ml calculated would be too high or too low if you make this mistake. You intend to add 0.9 ml of diluent to each tube and 0.1 ml of culture. Instead, you add 0.5 ml of diluent to each tube and 0.1 ml of culture to the first tube. Then, you make a serial dilution of 0.1 ml into and from each tube as described. You prepare 0.9 ml of diluents in each tube. You add 0.1 ml of culture (from the overnight culture provided) to every tube. You add 0.9 ml of diluent to each tube. You add 0.1 ml of…

The plate with 45 colonies was inocculated with 100 µL Volume of a 10^-8 Dilution. Given the formula OCD = CFU/V x D, what as the Original Colony Density?