A 20 mM solution of ethanol is diluted 1 in 8. The diluted ethanol solution was used for an alcoholdehydrogenase assay that had a total volume of 1.5 mL. 20 μL of alcoholdehydrogenase was used for the assay. The reaction was followed spectrophotometrically and a progress curve plotted from the spectrophotometric data. Using the progress curve, the initial velocity was calculated as 0.30 AA/min. The assay was repeated as above, with a different volume of alcoholdehydrogenase. The initial velocity was 0.01 AA/s. What volume (in µL) of alcoholdehydrogenase was used for the assay? (do not include units in the answer

A 20 mM solution of ethanol is diluted 1 in 8. The diluted ethanol solution was used for an alcoholdehydrogenase assay that had a total volume of 1.5 mL. 20 μL of alcoholdehydrogenase was used for the assay. The reaction was followed spectrophotometrically and a progress curve plotted from the spectrophotometric data. Using the progress curve, the initial velocity was calculated as 0.30 AA/min. The assay was repeated as above, with a different volume of alcoholdehydrogenase. The initial velocity was 0.01 AA/s. What volume (in µL) of alcoholdehydrogenase was used for the assay? (do not include units in the answer

Principles of Instrumental Analysis

7th Edition

ISBN:9781305577213

Author:Douglas A. Skoog, F. James Holler, Stanley R. Crouch

Publisher:Douglas A. Skoog, F. James Holler, Stanley R. Crouch

Chapter29: Supercritical Fluid Chromatography And Extraction

Section: Chapter Questions

Problem 29.7QAP

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Earth, a CHEM 40.1 student, extracted a mixture of pigments from the roots of Rubiatinctorum, commonly known as rose madder. Earth spotted the root extract on twohydrophobic silica gel plates. He then placed each plate in separate developing chambers,one containing solvent system WHITE and the other housing solvent system MIX. Earthallowed the chromatograms to develop for around five minutes. Afterwards, he removedthe plates from the containers and placed them inside an iodine chamber. Thechromatograms resulting from the TLC experiment after visualization are illustrated asfollows. The distances travelled by each spot and by the solvents were measured andrecorded in the displayed table.
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Q) water hardness of each trial and average ppm with calculation, please. Hard Water Trial 1 Hard Water Trial 2 Hard Water Trial 3 Initial Syringe Reading 1.0ml 1.0ml 1.0ml Final Syringe Reading 0.88ml 0.84ml 0.85ml Volume of EDTA Consumed 0.12ml 0.16ml 0.15ml Water Hardness ppm CaCO3 Average ppm
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