A biochemist makes up 95 mL of a 0.12 M solution of histidine at a pH of 5.6. She then adds 60 mL of 0.10 M HCI. What is the pH of the resulting solution?

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Chapter1: Biochemistry: An Evolving Science
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The amino acid histidine has ionizable groups with pKa values of 1.8, 6.0, and 9.2, as shown.
COOH
I
+
H₂N-CH
CH₂ H
-N
2
H
CH
lonizable -COOH
group
COO™
I
H₂N-CH
1.8
pk,
CH ₂
-COO™
H
-N
CH
-HisH+
H₂N-CH
6.0
COO™
pk ₂
CH, H
-N
H
-His
CH
-NH;
H₂N-CH
9.2
pk3
COO™
=
CH₂ H
-NH₂
CH
A biochemist makes up 95 mL of a 0.12 M solution of histidine at a pH of 5.6. She then adds 60 mL of 0.10 M HCl. What is the
pH of the resulting solution?
Transcribed Image Text:The amino acid histidine has ionizable groups with pKa values of 1.8, 6.0, and 9.2, as shown. COOH I + H₂N-CH CH₂ H -N 2 H CH lonizable -COOH group COO™ I H₂N-CH 1.8 pk, CH ₂ -COO™ H -N CH -HisH+ H₂N-CH 6.0 COO™ pk ₂ CH, H -N H -His CH -NH; H₂N-CH 9.2 pk3 COO™ = CH₂ H -NH₂ CH A biochemist makes up 95 mL of a 0.12 M solution of histidine at a pH of 5.6. She then adds 60 mL of 0.10 M HCl. What is the pH of the resulting solution?
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