A cloned Bam HI DNA fragment, 3.0 kb long, was isolated in preparation for sequence determination. First, a map of restriction endonuclease cleavage sites had to be prepared. Cleavage by each of the indicated enzymes generated fragments of the sizes shown on the three agarose electrophoresis gel figures below; the gels are running from top to bottom. On the diagram below those, show the relative positions of EcoR I and Hpa II cleavage sites and the distances (in kb) between them. Explain your reasoning – is your placement of the cleavage sites the only one that will work? Objective: Using DNA fragment length and other techniques to deduce the placement ofcleavage sites and replication centers.1. A cloned Bam HI DNA fragment, 3.0 kb long, was isolated in preparation for sequencedetermination. First, a map of restriction endonuclease cleavage sites had to beprepared. Cleavage by each of the indicated enzymes generated fragments of the sizesshown on the three agarose electrophoresis gel figures below; the gels are running fromtop to bottom. On the diagram below those, show the relative positions of EcoR I andHpa II cleavage sites and the distances (in kb) between them. Explain your reasoning -is your placement of the cleavage sites the only one that will work?ECORIBamHI1.7КВ0.9KB0.4KBECORIHpall-3.0kb-1.2KB0.9KB10.5KB0.4KBHpallBamHI1.6KB1.4KB

Restriction mapping is done by analysing and arranging the fragments that are generated by the use…

Answered: A cloned Bam HI DNA fragment, 3.0 kb…

Human Anatomy & Physiology (11th Edition)

11th Edition

ISBN:9780134580999

Author:Elaine N. Marieb, Katja N. Hoehn

Publisher:Elaine N. Marieb, Katja N. Hoehn

Chapter1: The Human Body: An Orientation

Section: Chapter Questions

Problem 1RQ: The correct sequence of levels forming the structural hierarchy is A. (a) organ, organ system,...

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A cloned Bam HI DNA fragment, 3.0 kb long, was isolated in preparation for sequence determination. First, a map of restriction endonuclease cleavage sites had to be prepared. Cleavage by each of the indicated enzymes generated fragments of the sizes shown on the three agarose electrophoresis gel figures below; the gels are running from top to bottom. On the diagram below those, show the relative positions of EcoR I and Hpa II cleavage sites and the distances (in kb) between them. Explain your reasoning – is your placement of the cleavage sites the only one that will work?

Objective: Using DNA fragment length and other techniques to deduce the placement of
cleavage sites and replication centers.
1. A cloned Bam HI DNA fragment, 3.0 kb long, was isolated in preparation for sequence
determination. First, a map of restriction endonuclease cleavage sites had to be
prepared. Cleavage by each of the indicated enzymes generated fragments of the sizes
shown on the three agarose electrophoresis gel figures below; the gels are running from
top to bottom. On the diagram below those, show the relative positions of EcoR I and
Hpa II cleavage sites and the distances (in kb) between them. Explain your reasoning -
is your placement of the cleavage sites the only one that will work?
ECORI
BamHI
1.7КВ
0.9KB
0.4KB
ECORI
Hpall
-3.0kb-
1.2KB
0.9KB
10.5KB
0.4KB
Hpall
BamHI
1.6KB
1.4KB

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