A phagehunter performs a spot titer using standard techniques (3 ul of each dilution spotted to lawn) of a lysate obtained from an optimum webbed plate experiment. The phagehunter counts 11 plaques on the 106 dilution spot. Which of the following scenarios is the best choice for the phagehunter to do next? The phagehunter has not achieved a high enough titer lysate to move forward with characterization experiments, so they should go back to the pick-a-plaque experiment. The phagehunter has not achieved a high enough titer lysate to move forward with characterization experiments, so they should try to make more optimum webbed plates. The phagehunter has achieved a high enough titer lysate to move forward with characterization experiments. The phagehunter has not achieved a high enough titer lysate to move forward with characterization experiments, so they should adopt a phage from direct isolation.

Basic Clinical Laboratory Techniques 6E
6th Edition
ISBN:9781133893943
Author:ESTRIDGE
Publisher:ESTRIDGE
Chapter4: Basic Immunology And Immunohematology
Section4.3: Tests For Rheumatoid Factors
Problem 2.1CS
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A phagehunter performs a spot titer using standard techniques (3 ul of each dilution spotted to lawn) of a lysate obtained from an optimum webbed plate experiment. The
phagehunter counts 11 plaques on the 106 dilution spot. Which of the following scenarios is the best choice for the phagehunter to do next?
The phagehunter has not achieved a high enough titer lysate to move forward with characterization experiments, so they should go back to the pick-a-plaque experiment.
O The phagehunter has not achieved a high enough titer lysate to move forward with characterization experiments, so they should try to make more optimum webbed
plates.
O
The phagehunter has achieved a high enough titer lysate to move forward with characterization experiments.
O The phagehunter has not achieved a high enough titer lysate to move forward with characterization experiments, so they should adopt a phage from direct isolation.
Transcribed Image Text:A phagehunter performs a spot titer using standard techniques (3 ul of each dilution spotted to lawn) of a lysate obtained from an optimum webbed plate experiment. The phagehunter counts 11 plaques on the 106 dilution spot. Which of the following scenarios is the best choice for the phagehunter to do next? The phagehunter has not achieved a high enough titer lysate to move forward with characterization experiments, so they should go back to the pick-a-plaque experiment. O The phagehunter has not achieved a high enough titer lysate to move forward with characterization experiments, so they should try to make more optimum webbed plates. O The phagehunter has achieved a high enough titer lysate to move forward with characterization experiments. O The phagehunter has not achieved a high enough titer lysate to move forward with characterization experiments, so they should adopt a phage from direct isolation.
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