A single UASG regulates the expression of threegenes, all of which are adjacent: GAL7 and GAL10 asdescribed in Problem 8, and also GAL1.a. Would you expect these genes to be transcribedinto individual transcripts, or to be cotranscribed asone mRNA? Explain.b. How could you determine experimentallywhether each gene is transcribed separately orinstead that the three are cotranscribed into asingle mRNA?c. GAL1 and GAL10 are not only adjacent to eachother, but also are transcribed divergently with asingle UASG between them. Describe experimentsusing GFP and RFP transgenes that would allowyou to determine which of the four GAL4 bindingsites in this UASG element is (are) important for thetranscription of GAL1 and/or GAL10.

Question

A single UASG regulates the expression of three
genes, all of which are adjacent: GAL7 and GAL10 as
described in Problem 8, and also GAL1.
a. Would you expect these genes to be transcribed
into individual transcripts, or to be cotranscribed as
one mRNA? Explain.
b. How could you determine experimentally
whether each gene is transcribed separately or
instead that the three are cotranscribed into a
single mRNA?
c. GAL1 and GAL10 are not only adjacent to each
other, but also are transcribed divergently with a
single UASG between them. Describe experiments
using GFP and RFP transgenes that would allow
you to determine which of the four GAL4 binding
sites in this UASG element is (are) important for the
transcription of GAL1 and/or GAL10.

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