A student researcher performed a chromatographic separation of caffeine and aspartame with methanol as the mobile phase using a C1g column. The retention times for methanol (tm), caffeine (tc), and aspartame (ta) were 34.9 s, 92.8 s, and 153.5 s, respectively. What is the separation factor (relative retention), a , for this column? a = 2.05 Calculate the hypothetical retention times for caffeine and aspartame if their retention factors were reduced by 25.0%. Assume the mobile phase retention time (tm = 34.9 s) remains constant. tc = S tĄ =

Question

What is tC?

What is tA?

A student researcher performed a chromatographic separation of caffeine and aspartame with methanol as the mobile phase
using a C1g column. The retention times for methanol (tm), caffeine (tc), and aspartame (ta) were 34.9 s, 92.8 s, and
153.5 s, respectively.
What is the separation factor (relative retention), a , for this column?
a =
2.05
Calculate the hypothetical retention times for caffeine and aspartame if their retention factors were reduced by 25.0%.
Assume the mobile phase retention time (tm = 34.9 s) remains constant.
tc =
S
tĄ =
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Transcribed Image Text

A student researcher performed a chromatographic separation of caffeine and aspartame with methanol as the mobile phase using a C1g column. The retention times for methanol (tm), caffeine (tc), and aspartame (ta) were 34.9 s, 92.8 s, and 153.5 s, respectively. What is the separation factor (relative retention), a , for this column? a = 2.05 Calculate the hypothetical retention times for caffeine and aspartame if their retention factors were reduced by 25.0%. Assume the mobile phase retention time (tm = 34.9 s) remains constant. tc = S tĄ =

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