(a) Transcription components RNA polymerase core enzyme o subunit NTPS DNA Promoter Gene (b) Template binding and initiation of transcription Coding strand 5' Nascent 5' RNA Template strand (c) Chain elongation o dissociates 5 Growing RNA transcript FIGURE 13.9 The early stages of transcription in bacteria, showing (a) the components of the process; (b) template binding at the -10 site involving the sigma subunit of RNA polymerase and subsequent initiation of RNA synthesis; and (c) chain elongation, after the o subunit has dissociated from the transcription complex and the enzyme moves along the DNA template.
Bacterial Genomics
The study of the morphological, physiological, and evolutionary aspects of the bacterial genome is referred to as bacterial genomics. This subdisciplinary field aids in understanding how genes are assembled into genomes. Further, bacterial or microbial genomics has helped researchers in understanding the pathogenicity of bacteria and other microbes.
Transformation Experiment in Bacteria
In the discovery of genetic material, the experiment conducted by Frederick Griffith on Streptococcus pneumonia proved to be a stepping stone.
Plasmids and Vectors
The DNA molecule that exists in a circular shape and is smaller in size which is capable of its replication is called Plasmids. In other words, it is called extra-chromosomal plasmid DNA. Vectors are the molecule which is capable of carrying genetic material which can be transferred into another cell and further carry out replication and expression. Plasmids can act as vectors.
I'm studying Transcription in bacteria, my textbook writes that:
"the holoenzyme “explores” a length of DNA until it encounters a promoter region and binds there to about 60
my question is what proteins or factors unwind the helix?
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