A type of phosphatase can be assayed using p-nitrophenyl phosphate. The assay is performed at pH 7.8, which is the optimum pH for this enzyme. The initial velocity measured at the highest substrate concentration was 0.025 ΔA400nm/minute. Convert this velocity to a change in concentration/minute (μM/min). Use 18,380M^-1cm^-1 for the extinction coefficient.

A type of phosphatase can be assayed using p-nitrophenyl phosphate. The assay is performed at pH 7.8, which is the optimum pH for this enzyme. The initial velocity measured at the highest substrate concentration was 0.025 ΔA400nm/minute. Convert this velocity to a change in concentration/minute (μM/min). Use 18,380M^-1cm^-1 for the extinction coefficient.

Introduction to General, Organic and Biochemistry

11th Edition

ISBN:9781285869759

Author:Frederick A. Bettelheim, William H. Brown, Mary K. Campbell, Shawn O. Farrell, Omar Torres

Publisher:Frederick A. Bettelheim, William H. Brown, Mary K. Campbell, Shawn O. Farrell, Omar Torres

Chapter23: Enzymes

Section: Chapter Questions

Problem 23.14P

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