A yeast disaccharidase can hydrolyze sucrose and maltose according to the following table: Use Lineweaver-Burk representation to calculate the respective KMs and maximum speeds for each substrate under the conditions of the experiment. Saccharose(mM) Glucose released (µmol10 min) Maltose (mM) Glucose released (µmol10 min) 5 40 5 48 10 60 10 80 20 80 20 120 50 100 50 172 100 109 100 200

Basic Clinical Laboratory Techniques 6E
6th Edition
ISBN:9781133893943
Author:ESTRIDGE
Publisher:ESTRIDGE
Chapter6: Basic Clinical Chemistry
Section6.5: Blood Glucose And Hemoglobin A1c
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A yeast disaccharidase can hydrolyze sucrose and maltose according to the following table:

Use Lineweaver-Burk representation to calculate the respective KMs and maximum speeds for each substrate under the conditions of the experiment.

Saccharose(mM)

Glucose released (µmol10 min)

Maltose (mM)

Glucose released (µmol10 min)
5 40 5 48
10 60 10 80
20 80 20 120
50 100 50 172
100 109 100 200
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