A yeast disaccharidase can hydrolyze sucrose and maltose according to the following table: Use Lineweaver-Burk representation to calculate the respective KMs and maximum speeds for each substrate under the conditions of the experiment. Saccharose(mM) Glucose released (µmol10 min) Maltose (mM) Glucose released (µmol10 min) 5 40 5 48 10 60 10 80 20 80 20 120 50 100 50 172 100 109 100 200
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A yeast disaccharidase can hydrolyze sucrose and maltose according to the following table:
Use Lineweaver-Burk representation to calculate the respective KMs and maximum speeds for each substrate under the conditions of the experiment.
Saccharose(mM) |
Glucose released (µmol10 min) |
Maltose (mM) |
Glucose released (µmol10 min) |
5 | 40 | 5 | 48 |
10 | 60 | 10 | 80 |
20 | 80 | 20 | 120 |
50 | 100 | 50 | 172 |
100 | 109 | 100 | 200 |
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