a. Describe the process of protein folding and quality control. What are the steps and major proteins involved in folding, quality control, and the outcome if a protein is not properly folded? What is ED

Biology: The Dynamic Science (MindTap Course List)
4th Edition
ISBN:9781305389892
Author:Peter J. Russell, Paul E. Hertz, Beverly McMillan
Publisher:Peter J. Russell, Paul E. Hertz, Beverly McMillan
Chapter3: Biological Molecules: The Carbon Compounds Of Life
Section: Chapter Questions
Problem 8TYK: The first and major effect in denaturation of proteins is that: a. peptide bonds break. b. helices...
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2. You are studying protein folding and quality control. You hypothesize that protein,
ERRP, is an ER resident protein that is required for the process of protein folding and
quality control. Answer the following questions and then use the answers to help you to
design an experiment to test your hypothesis.
a. Describe the process of protein folding and quality control. What are the steps
and major proteins involved in folding, quality control, and the outcome if a
protein is not properly folded?
b. What is an ER resident protein? Provide examples of ER resident proteins and
explain the cellular mechanism used to localize ER resident proteins to the ER;
how do these proteins get to the ER? how do they stay in the ER? How are they
sent back to the ER if they accidentally get shipped to the Golgi?
c. You decide to mutate the gene that encodes ERRP so that it is localized to the
Cytoplasm instead of the ER so you can observe the effects on protein folding
and quality control. What kind of mutation would you make that would result in
the protein localizing to the cytoplasm? Why would this mutation result in
localizing ERRP to the cytoplasm?
d. You decide to make a second mutation to localize the protein to the Nucleus
rather than the ER. What kind of mutation would result in localization of the
protein to the Nucleus? Why would this mutation result in localizing ERRP to the
nucleus?
е.
How can you use these mutants to test your hypothesis? Remember your
hypothesis was - ERRP is an ER resident protein that is required for protein
folding and quality control.
Transcribed Image Text:2. You are studying protein folding and quality control. You hypothesize that protein, ERRP, is an ER resident protein that is required for the process of protein folding and quality control. Answer the following questions and then use the answers to help you to design an experiment to test your hypothesis. a. Describe the process of protein folding and quality control. What are the steps and major proteins involved in folding, quality control, and the outcome if a protein is not properly folded? b. What is an ER resident protein? Provide examples of ER resident proteins and explain the cellular mechanism used to localize ER resident proteins to the ER; how do these proteins get to the ER? how do they stay in the ER? How are they sent back to the ER if they accidentally get shipped to the Golgi? c. You decide to mutate the gene that encodes ERRP so that it is localized to the Cytoplasm instead of the ER so you can observe the effects on protein folding and quality control. What kind of mutation would you make that would result in the protein localizing to the cytoplasm? Why would this mutation result in localizing ERRP to the cytoplasm? d. You decide to make a second mutation to localize the protein to the Nucleus rather than the ER. What kind of mutation would result in localization of the protein to the Nucleus? Why would this mutation result in localizing ERRP to the nucleus? е. How can you use these mutants to test your hypothesis? Remember your hypothesis was - ERRP is an ER resident protein that is required for protein folding and quality control.
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