Absorbance Absorbance Questions 2-4 refer to the absorption spectrum for a 0.80 M solution of metal M2 and a calibration curve for the metal M2 collected in a 1.5 cm cuvette, shown in Figures 2 and 3. 60 9 0 0.4 0.4 0.3 0.2 0.2 000 550 1.2 50 0.8 009 0.4 000 0.2 Wavelength (nm) M2+] (M) Figure 2. Absorption spectrum of 0.80 M M2+ Figure 3. Calibration curve for M2 2. At what wavelength was the calibration curve obtained? Use the data from both graphs to explain your choice. 3. Would the value of the molar absorptivity (E) be greater, lesser or equal for M for the absorbance of light at 400. nm vs. 650. nm. Use the data from Figure 2 to explain your choice 4. Using the calibration curve in Figure 3: A sample of a solution of unknown concentration of M2* has an absorbance reading that is "off scale" because the absorbance was measured to be above 0.8. You dilute this sample by adding 5.0 mL of water to 1.0 mL of the solution of M2+. The absorbance of the diluted solution was determined to be 0.35 at the same wavelength used for the calibration curve. What the concentration of M2, in M, of the original undiluted sample?
Absorbance Absorbance Questions 2-4 refer to the absorption spectrum for a 0.80 M solution of metal M2 and a calibration curve for the metal M2 collected in a 1.5 cm cuvette, shown in Figures 2 and 3. 60 9 0 0.4 0.4 0.3 0.2 0.2 000 550 1.2 50 0.8 009 0.4 000 0.2 Wavelength (nm) M2+] (M) Figure 2. Absorption spectrum of 0.80 M M2+ Figure 3. Calibration curve for M2 2. At what wavelength was the calibration curve obtained? Use the data from both graphs to explain your choice. 3. Would the value of the molar absorptivity (E) be greater, lesser or equal for M for the absorbance of light at 400. nm vs. 650. nm. Use the data from Figure 2 to explain your choice 4. Using the calibration curve in Figure 3: A sample of a solution of unknown concentration of M2* has an absorbance reading that is "off scale" because the absorbance was measured to be above 0.8. You dilute this sample by adding 5.0 mL of water to 1.0 mL of the solution of M2+. The absorbance of the diluted solution was determined to be 0.35 at the same wavelength used for the calibration curve. What the concentration of M2, in M, of the original undiluted sample?
Principles of Instrumental Analysis
7th Edition
ISBN:9781305577213
Author:Douglas A. Skoog, F. James Holler, Stanley R. Crouch
Publisher:Douglas A. Skoog, F. James Holler, Stanley R. Crouch
Chapter15: Molecular Luminescence Spectrometry
Section: Chapter Questions
Problem 15.16QAP
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please answer question 2 and show work. thank you
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