Absorbance at Amax

Principles of Instrumental Analysis
7th Edition
ISBN:9781305577213
Author:Douglas A. Skoog, F. James Holler, Stanley R. Crouch
Publisher:Douglas A. Skoog, F. James Holler, Stanley R. Crouch
Chapter6: An Introduction To Spectrometric Methods
Section: Chapter Questions
Problem 6.18QAP
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Calculate the transmittance (in %) of an unknown concentration of a protein that was standardized using the following data:
concentration of the reference
Absorbance at Amax
protein (mg / mL)
1.00
0.247
3.00
0.559
5.00
0.768
7.00
0.997
9.00
1.243
Absorbance of the unknown protein: 0.728
Provide your answer using 3 significant figures.
Transcribed Image Text:Calculate the transmittance (in %) of an unknown concentration of a protein that was standardized using the following data: concentration of the reference Absorbance at Amax protein (mg / mL) 1.00 0.247 3.00 0.559 5.00 0.768 7.00 0.997 9.00 1.243 Absorbance of the unknown protein: 0.728 Provide your answer using 3 significant figures.
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