Acid phosphatases are an important group of enzymes that can be detected in human blood serum. Under slightly acidic conditions (pH 5.0), this group of enzymes can hydrolyze biological phosphate esters as follows: R-O-P-O;² + H 20 → R-OH + HO-P-O;² Acid phosphatases are produced and can be detected in erythrocytes, kidney, spleen, the liver, and prostrate gland. The enzyme from the prostrate gland is clinically important because an increased activity in the blood is frequently an indication of cancer of the prostrate gland. Tartrate ion can strongly inhibit the phosphatase from the prostrate gland, but not acid phosphatases from other tissues. How can you use the information above to develop a specific

Question
Acid phosphatases are an important group of enzymes that can be detected in human blood
serum. Under slightly acidic conditions (pH 5.0), this group of enzymes can hydrolyze
biological phosphate esters as follows:
R-O-P-O;² + H 20 → R-OH + HO-P-O;²
Acid phosphatases are produced and can be detected in erythrocytes, kidney, spleen, the liver,
and prostrate gland. The enzyme from the prostrate gland is clinically important because an
increased activity in the blood is frequently an indication of cancer of the prostrate gland.
Tartrate ion can strongly inhibit the phosphatase from the prostrate gland, but not acid
phosphatases from other tissues. How can you use the information above to develop a specific
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Transcribed Image Text

Acid phosphatases are an important group of enzymes that can be detected in human blood serum. Under slightly acidic conditions (pH 5.0), this group of enzymes can hydrolyze biological phosphate esters as follows: R-O-P-O;² + H 20 → R-OH + HO-P-O;² Acid phosphatases are produced and can be detected in erythrocytes, kidney, spleen, the liver, and prostrate gland. The enzyme from the prostrate gland is clinically important because an increased activity in the blood is frequently an indication of cancer of the prostrate gland. Tartrate ion can strongly inhibit the phosphatase from the prostrate gland, but not acid phosphatases from other tissues. How can you use the information above to develop a specific