After performing a Gram stain on a Gram negative bacteria, the specimen does not show pink or purple bacteria. Which of the following could notexplain why this occurred? Decolorizer was used after safranin Safranin was not used Crystal violet was not used The specimen was not heat-fixed
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After performing a
Decolorizer was used after safranin
Safranin was not used
Crystal violet was not used
The specimen was not heat-fixed
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- The first step in identifying an organism from a pure working culture is to perform a(n) __________ test. acid-fast staining negative staining endospore staining simple staining Gram stainThe decolorizing agent damages the peptidoglycan layer. Why is this important to consider when deciding how long to decolorize a specimen as part of the Gram stain procedure?Which of the following are true about a differential stain. There may be more than one answer! No chemicals are needed. More than one stain is used The acid fast stain is an example The Gram stain is an example.
- Describe the color and appearance of a Gram positive and Gram negative bacteria as it is treated at every step of the Gram staining procedureExplain how bacterial cells would look in the gram staining procedure if the following mistakes were made: a. Decolorizer left on too long b. Decolorizer not left on long enough c. Slide not heat-fixed before stainingThe unknown observations are shown as a mixed stain, gram positive, and gram negative. The next images shows the chart of what we can choose from for the gram positive bacterial stain. We are allowed to choose 4 test or stains that we would like to use to preform to identify the unknown.
- An acid-fast staining method was performed on a bacterial smear using the dyes carbolfuchsin and methylene blue. The resulting stained slide contained only cells that appeared blue when visualized using a microscope. Which of the following could explain why only blue cells were present? No acid-fast bacteria are present on the slide Methylene blue was not used Carbolfuchsin was not used Acid-alcohol was not usedWhich of the following would suggest your stock culture is contaminated? Which of the following would suggest your stock culture is contaminated? Gram stain indicates Gram-positive bacilli. Broth media become turbid. Gram stain indicates both Gram-positive cocci and Gram-negative bacilli. Colony morphology is uniform on the streak isolation plate. Gram stain indicates Gram-positive staphylococci.Describe one error in the staining procedure that would result in a Gram-positive bacterium appearing pink.
- Before viewing a specimen of pigmented bacteria on a slide under the light microscope, which of the following usually needs to happen (and why)? Staining (to increase magnification). Heat, radiation or antimicrobial chemical treatment (to kill the bacteria for safe observation). Add immersion oil (to increase resolution). Add a cover slip (to reduce contamination). The viewing chamber needs to be flushed of air (to create a vacuum).After performing the gram stain you observe your result as gram negative. To help confirm this result what type of method (besides the gram stain) could you perform? Please explain why, the result you would want to observe and the interpretation of the result.Out of the three gram-positive bacteria (Micrococcus luteus, Staphylococcus aureus, and Staphylococcus epidermidis). Which of the following test(s) will help me identify the unknown gram-positive bacteria? Gram Stain Wet Mount (motility testing) Endospore Staining Acid Fast Staining Differential & Selective Media (MacConkey, PEA, EMB, MSA Agar, Blood Agar, etc.) Oxidative-Fermentative Test Carbohydrate Fermentation MR-VP Testing Indole Production Citrate Utilization Triple Sugar Iron Testing Catalase Test