After reacting two liquid reactants with eachother and allowing them to reflux for 2 hours. There was very poor percentage yield of product. Why?
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After reacting two liquid reactants with eachother and allowing them to reflux for 2 hours. There was very poor percentage yield of product. Why?
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- Why should the purified compound at the well (Buchner funnel) not be washed with a larger amount of pure solvent?Which of the three methods, (Use of Separatory Funnel, Decantation or Use of Pasteur Pipette) do you believe is most suitable for transferring large volumes of liquid mixtures and why?Why is necessary to perform more than one extraction to obtain all the compound from the aqueous to the organic phase?
- Q1: Give one lysis buffer that is commonly used for western blotting experiments and include its components Q3: To make sure that you used a similar amount of samples, what important step should be done before proceeding the electrophoresis stage? Q4. Why is it necessary to store the prepared lysates in a very low temperature?In the organic chemistry experiment of an Extraction of crude napthalene and benzoic acid dissolved in ethyl acetate solvent. Why are multiple small extractions better than 1 large extraction? Explain. Prove this with mathematically.Explain why aqueous permanganate must be freshly standardised before use in the experiment Determination of sodium percarbonate in a commercial detergent .
- Briefly explain why should the flask be stoppered after the addition of KI in the standardization of sodium thiosulfate VS.Propose an experimental design on the separation of components of the mixture of o-nitrophenol and p-nitrophenol., provide a schematic diagram of the step-by-step procedureSodium hydroxide was used to help separate benzoic acid and fluorenone because deprotonated benzoic acid (sodium benzoate) favorably partitions into the aqueous phase (and fluorenone does not). Would this procedure have worked without the sodium hydroxide (using simply neutral water)? Why or why not?
- TLC, a powerful analytical tool, can be used to monitor the progress of reactions. The synthesis of ethyl-3-coumarincarboxylate can be monitored by TLC by displaying the starting aldehyde 1, and coumarin product 2, which have very distinct Rf values (Hint: Think about the polarity of compounds 1 and 2 in terms of their abilities to H-bonds to silica gel). What can be determine about the progress of the reaction from analysis of the TLC shown below?Explain the function of using dichloromethane after the steam extraction of Eugenol. Also discuss if there are any differences and which ones in soxhlet extraction and steam drag.In the organic chemistry experiment of an Extraction of crude napthalene-benzoic acid dissolved in ethyl acetate solvent. Why are multiple small extractions better than 1 large extraction?. Prove this with mathematically. Kindly use math and numbers! showing everything.