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- Enumerate the advantages derived from staining bacteria. In Gram staining method, list the reagents and state their role for each step of the procedure? In preparing bacterial smear, why do we need to pass the slide over the flame?ou make a smear of E. coli and then simple stain the slide with methylene blue. If you failed to use correct aseptic technique by not sterilizing the loop before picking up the bacterial sample for the smear, what results may be seen under the microscope?In Gram staining method, list the reagents and state their role for each step of the procedure? In preparing bacterial smear, why do we need to pass the slide over the flame?
- What cannot be determined using the details of Gram staining process? Question 7 options: bacterial shape cell wall structure antibiotic treatment motility structuremicrobial sensivity lab: in the procedure used to test bacterial growth against various temperatures (incubator, room, refrigerator, freezer), why should efforts be made to inoculate each tube with the same number of bacteria?Dichotomous key:b. Based on the key, bacteria of which genus would appear purple after a Gram-staining test? please refer to the attached photo.
- Which of the following is similar to a block of highly purified “jello-like” substance that is not completely solid and has numerous pores throughout its structure? A. Micropipette b. Restriction enzymes c. Agarose d. Negative electrode please give correct answerWhich stain can be used to determine if bacteria are capsulated? Question options: flagella stain negative stain simple stain capsule stainYour instructor asks you to isolate and identify the organisms in an unknown culture. You find that the culture contains two gramnegative bacilli that produce swarming colonies. What biochemical test would you use to identify the bacilli? Justify your answer.
- Evaluate the following preparations in terms of providing informationon microbial size, shape, motility, and differentiation: spore stain,negative stain, simple stain, hanging drop slide, and Gram stain.Based from the results obtained from Gram staining, explain why bacterial cells retained or changed their color. Briefly explain the principle behind the procedure. Cite advantages of differential staining method (Gram staining and endospore staining) over simple staining only. List other staining methods aside from those you performed in the laboratory. conclusion:Describe in detail all the steps needed to determine whether an Unknown bacteria has a Gram-positive or Gram-negative cell wall using the Gram stain. (You can describe the method presented in the Exercise 3-6 Gram stain videos or in the Lab Manual.) 1. Start by describing the method of transferring the bacteria onto the microscope slide, then describe heat-fixing the emulsion of the Unknown bacteria. (Do not describe all the steps needed to perform aseptic technique. Just say “Bacteria was added aseptically.”) 2. Describe the Gram stain in detail. (This description should include all the stains, how long the stain should set on slide, and Genus and species names of bacteria you are using as CONTROLS.)