An equilibrium dialysis study was carried out with a ligand binding receptor protein. The protein was present at a concentration of 0.325 µM. Consider the plot below showing data from the study and answer questions [Bound]/[Free] 40 g 20 10 0 0.2 0.4 0.6 0.8 1.0 [Bound] (uM) 1.2 1.4
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1) How many binding sites per molecule does the receptor have?
2) What type of cooperativity is observed with this binding protein?
a) No cooperativety
b) Positive homotropic cooperativity
c) Positive heterotropic cooperativity
d) Negative homotropic cooperativity
e) Negative heterotropic cooperativity
f) none of the above
![An equilibrium dialysis study was carried out with a ligand binding receptor protein. The protein
was present at a concentration of 0.325 µM. Consider the plot below showing data from the
study and answer questions
[Bound]/[Free]
8
20
10-
0.2 0.4 0.6 0.8 1.0 1.2
[Bound] (uM)
1.4](/v2/_next/image?url=https%3A%2F%2Fcontent.bartleby.com%2Fqna-images%2Fquestion%2F05dea9d6-4326-4c2b-a236-db1a7bc75ad8%2F76a3a668-6081-4c82-bb69-74bb1a61f645%2Fkj5jnjc_processed.jpeg&w=3840&q=75)
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could the answer be no cooperativity as well?
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