As a molecular biologist and horticulturist specializing in snapdragons, you have decided that you need tomake a genomic library to characterize the flowercolor genes of snapdragons.a. How many genomic equivalents would you like tohave represented in your library to be 95% confident of having a clone containing each gene inyour library?
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As a molecular biologist and horticulturist specializing in snapdragons, you have decided that you need to
make a genomic library to characterize the flower
color genes of snapdragons.
a. How many genomic equivalents would you like to
have represented in your library to be 95% confident of having a clone containing each gene in
your library?
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- . The position of the gene for the protein actin in the haploid fungus Neurospora is known from the complete genome sequence. If you had a slow-growing mutant thatyou suspected of being an actin mutant and you wantedto verify that it was one, would you (a) clone the mutantby using convenient restriction sites flanking the actingene and then sequence it or (b) amplify the mutantgene by using PCR and then sequence it?a To make a genomic library useful for sequencingan entire genome, why would you ordinarily fragment the genomic DNA by mechanical shearingforces like sonication rather than by cutting theDNA with a restriction enzyme?b. Suppose that you wanted to make a genomic library to determine the complete sequence of anewly discovered organism’s genome, but you didnot have a sonicator readily available. Explain howyou could nonetheless use two or more restrictionenzymes to make libraries whose clones could besequenced so that a computer could assemble thegenomic sequence.c. Suppose you only had a single restriction enzymeavailable, and you want to make a single genomiclibrary from which you could assemble the genomic sequence. How might you be able to achievethis goal? (Hint: See Problem 9.) To make this library, would it be preferable to use a restriction enzyme that recognizes a 4-base, 6-base, or 8-basesequence of DNA?Pedigree Analysis Is a Basic Method in Human Genetic: What does OMIM stand for? What kinds of information are in this database?
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- You are using the restriction enzyme HAEIII to digest different samples of the taster gene isolated from cheek cells of different people and amplified by PCR. When viewing the bands on the electrophoresis gel, one would expect that a taster (homozygote) would have---------band(s), whereas a carrier (heterozygote) would show--------band(s), and a non-taster would show------band(s).Hi, I would like to know which program is used for the graphical presentation of the results of a meta-analysis of genome-wide linkage scans?Which goals of the Human Genome Project do you think are themost important? Why? Discuss the types of ethical problems thatmight arise as a result of identifying all of our genes.
- Scientists are interested in cloning an extinct animal called the gastricbrooding frog. This strange frog swallows its eggs and broods itsyoung within its stomach. So far, scientists have successfully usedcloning to make an embryo of the frog, but they have yet to raise oneto maturity. What steps might the scientists have used to clone thisextinct species? Why was it important for scientists to determinebefore the experiments that the great barred frog is a close relative tothe gastric brooding frog?Next-generation sequencing reveals that six new mutations have occurred in the coding regions of genes in an individual diploid fly. If the coding regions of this fly comprise 100 million nucleotides per haploid genome, what is the mutation rate per nucleotide?In order to make a plant gene sequence functional when added to a bacterial genome, what kinds of changes would you have to make to the plant gene sequence?