Asked Nov 13, 2019

Asparagine and glutamine residues in proteins are deamidated in an aqueous solution to yield aspartate and glutamate, respectively, and ammonium.  Proteins undergoing deamidation of one or more of their Asn or Gln groups have been detected by isoelectric focusing.  Why would isoelectric focusing be effective in separating deamidated from non-deamidated protein?  Compare the relative pI values of the deamidated and non-deamidated proteins.


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Deamidation is the conversion of amide group to another functional group. Si...

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