Based on the following titration table of Glycine, would you be able to recreate the same for Histidine?

Biochemistry
9th Edition
ISBN:9781319114671
Author:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.
Publisher:Lubert Stryer, Jeremy M. Berg, John L. Tymoczko, Gregory J. Gatto Jr.
Chapter1: Biochemistry: An Evolving Science
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Based on the following titration table of Glycine, would you be able to recreate the same for
Histidine?
Increasing pH –
Glicina
H
H-N-CH,-COOH = H-N-CH,-CO0- = H-N-CH,-coo-
H
H
Net charge:
+1
-1
pH
COOH-pKa = 2.3
NH3-pKa = 9.6
CN
ΔΕ
+1
+1
2.3
(0.5*-1)+(0.5*0)=-0.5
+1
+0.5
0.5
9.6
-1
(0.5*+1)+(0.5*0)=+0.5
-0.5
1.5
14
-1
-1
2
Given that the pKas for Histidine are the following:
pka1 = 1.82
pKa2 = 9.17
pКа3 %3D 6.00
pl = 7.59
Transcribed Image Text:Based on the following titration table of Glycine, would you be able to recreate the same for Histidine? Increasing pH – Glicina H H-N-CH,-COOH = H-N-CH,-CO0- = H-N-CH,-coo- H H Net charge: +1 -1 pH COOH-pKa = 2.3 NH3-pKa = 9.6 CN ΔΕ +1 +1 2.3 (0.5*-1)+(0.5*0)=-0.5 +1 +0.5 0.5 9.6 -1 (0.5*+1)+(0.5*0)=+0.5 -0.5 1.5 14 -1 -1 2 Given that the pKas for Histidine are the following: pka1 = 1.82 pKa2 = 9.17 pКа3 %3D 6.00 pl = 7.59
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