Based on the TLC plate shown below, what was the error made? O The eluent used was too polar The sample spotted in lane 3 was too concentrated Multiple compounds were spotted in lane 3 O There is no error in this plate
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- i need to caluclate a rf value for the tlc plate and i really need help with showing the steps please! Reduction of 4-Nitrobenzaldehyde to 4-Nitrobenzyl Alcohol and Thin Layer ChromatographyThin Layer Chromatography why the non-polar compounds move up the plate most rapidly (higher Rf value), whereas polar substances travel up the TLC plate slowly or not at all (lower Rf value)? Please help me explain this question! Thank you so much!Two compounds A and B from GC on two open tubular columns contains a XY-1 (100% methyl) and a XY-2 (50% methyl, 50% phenyl), with the same diameter, thickness and length under the same experimental conditions. The retention times and peak widths are show in the Table 1.Hint: phenyl groups interact more strongly with polar groups than methyl groups. Which compound is more polar? Explain your answer
- You and another student were each given an unknown compound. Both samples contained colorless material. You each used the same brand of commercially prepared TLC plate and developed the plates using the same solvent. Each of you obtained a single spot of Rf = 0.75. Were the two samples necessarily the same substance? How could you prove unambiguously that they were ily identical using TLC?The three TLC plates below show the analysis of the same mixture of two compounds. One plate was developed in 100% DCM, one in 90:10 DCM/MeOH, and one in 80:20 DCM/MeOH. Determine which plate was developed in which solvent.The following Rf values were computed from a normal phase chromatography experiment. Arrange them from most polar to least polar. I - 0.25 II - 0.84 III - 0.56 IV- 0.67
- An unknown mixture was analyzed with TLC on a silica gel plate. Development gave two spots with Rf values of 1.0 and 0.0 respectively.a) Can you say for sure how many compounds are present in the mixture? If so, how many are there? Explain. b) If not, what additional TLC experiment(s) might be performed to determine how many components are present? Explain.A student spots an unknown sample on a TLC plate and develops it in pentane solvent. Only one spot, for which the Rf value is 0.05, is observed. Is the un-known material a pure compound? What can be done to verify the purity of the sample?A student spots an unknown sample on a TLC plate and develops it in dichloromethane solvent. Only one spot, for which the Rf value is 0.95, is observed. Does this indicate that the unknown material is a pure compound? What can be done to verify the purity of the sample?
- How does increasing the polarity of the developing solvent affect the Rf of a compound on a silica gel TLC plate?You spotted a mixture of three compounds, labeled A-C, on a silica plate. The resulting thin layer chromatography (TLC) plate with a mobile solvent mixture of 95 % hexanes and 5 % ethyl acetate is shown below. Which of the spots traveled the furthest up the TLC plate corresponding to spot 3?In TLC, why does the developing solvent 3:1:1 n-butanol/acetic acid/water climb up the TLC plate so slowly?