Before developing a TLC plate you should _______. a. Make sure to mark the origin with a pen b. Make sure to mark the origin with a pencil  c. Make sure the filter is all the way around the inside of the developing chamber

Q: How do i know my starting material was fully consumed using a TLC plate comparing the raction…

A: TLC means thin layer chromatography is a chromatographic technique used for the separation of…

Q: d) In a typical HPLC column, 150 atm of pressure is required to push the mobile phase through the…

A: In this question, we have to find out the correct answer of given problem by the help of the concept…

Q: What happens when the level of the eluent solution in the developing chamber (beaker) is adjusted to…

A:

Q: economical purification technique that ca n be pure material? O Distillation O Another extraction…

A:

Q: Study figures 31-6 a & b in chapter 31 (page 863) of your prescribed textbook, and draw separation…

A: 1

Q: What happens if the spots are made too large when preparing a TLC plate for development?

A: This question is related to chromatography.

Q: What would happen if we use longer columns and select adsorbing materials with 200-400 mesh sizes…

A: Solution is given in the following steps:

Q: How can you vary the retention factor and selectivity factor?

A: Retention factor is a variable which indicates the time duration a component spends in the…

Q: Can an HPLC run be stopped during operation without impairing the separation

A: HPLC- High performance liquid chromatoghraphy It consists of two phase .One is stationary phase and…

Q: in what situation does one use  thin and/or column chromatography? You can any of theme for anything…

A: Chromatography is an analytical technique to separate mixtures into it's components.  It is also…

Q: In reverse phase chromatography which analyte will elute first, most polar or least polar?

A: In reverse phase chromatography, polar solvent is used as a mobile phase.

Q: Why is splitless injection used with purge and trap sample preparation?

A: The collection of all of the analyte from the unknown and to inject whole of the analyte into the…

Q: Your lab-mate observes a large amount of streaking and blurring of their samples when they visualize…

A: It is the major problem that YTLC plate seems to be blurred in the UV light.  But you can overcome…

Q: What could happen if you used ink to draw on your baseline and letters on the TLC plate?

A: Pencil is always used to mark chromatography paper.

Q: Name 3 adsorbents used in TLC. Mention their use and properties.  Why is it important to use a…

A: 3 adsorbents used in TLC -  1. Silica gel : Silica gel is by far the most widely used adsorbent and…

Q: Why is it important to handle the TLC plate in such a way that your fingers do not come in contact…

A: Chromatography works on the principle that different compounds will have different solubilities and…

Q: How does temperature, flow rate of carrier gas, boiling point and column suupport affect gas…

A: Explanation. Retention time-     Retention time is the amount of time a compound spends on…

Q: if air can degrade a column and cause TC detector filaments to burn out, why do we use an air peak?

A: Interpretation -  If air can degrade a column and cause TC detector filaments to burn out, why do we…

Q: What should you do if there are insoluble impurities after heating up your sample in the…

A: A multiple choice question based on purification, which is to be accomplished.

Q: A small quantity of compound A was applied to the mid-point of the following TLC plate’s line of…

A:

Q: How resolution in HPLC analysis can be improved?

A: HPLC i.e  high-performance liquid chromatography is an analytical method which is being used to…

Q: What is a purification method that is more "green" than flash chromatography? (produces less waste).…

A:

Q: Fill in the blanks: is applied when TLC will be used to isolate compounds. It uses a plate.

A: Thin layer chromatography is a technique used to isolate non volatile mixtures.

Q: Give 2 examples of High performance liquid chromatography (HPLC) on how or when it is recommended to…

A: High performance liquid chromatography is the modification of liquid chromatography. Now a days it…

Q: Inches Centimeters 5 21 ● A B C S Inches Centimeters 2 Starting point ----+ Unknown

A: Here we have to calculate the Rf factor for different known ( A, B , C) species and also for an…

Q: High performance liquid chromatography can separate eluates even if they are not present in the UV…

A: The actual separation of each component in a sample is always carried inside a column and to be able…

Q: How is the retention factor calculated for a specific spot when doing the TLC analysis? O Distance…

A: We have to tell the process of calculating of retention factor for a particular spot. The definition…

Q: It refers to the general term of adsorbent media used to visualize chromogenic compounds. A.…

A: Chromatogram refers to the general term of adsorbent media used to visualize chromogenic compounds.…

Q: 1. a) "Solution 1" is made from the addition of water to a portion of the 0.0500 M  Fe3+ "…

A: Given, Molarity of prototype solution = 0.05M Fe3+. Volume of prototype solution = 10.0mL. Volume of…

Q: Explain why the indicators, red and blue,  are separated on the TLC plate during a chromatography…

A: Thin ayer chromatography is a chromatography technique, which is used to separate non-volatile…

Q: What error would occur if the Sep-Pak C18 cartridge (the nonpolar stationary phase) was not…

A: C-18 cartridges are columns that are filled with packing material that act as stationary phase. The…

Q: What is the retention factor (Rf) of the most polar and least polar spot present in the standard…

A: We have to give the retention factor (Rf) of the most polar and least polar spots present in the…

Q: Thin‑layer chromatography (TLC) can be used as a preliminary identification method for many…

A: Given that., Distance traveled by the spot from bottom = 3.52 cm Distance travel by mobile phase =…

Q: how to know if my product was pure usiing a TLC plate that has reaction mixture, co spot, and…

A: Thin layer Chromatography is also known as TLC which is a very useful tool to check a reaction is…

Q: if you wanted to makes the spots travel up the TLC plate further, what solvent ration would you…

A: You know that ethyl acetate is a polar solvent while hexane is a non-polar solvent.

Q: what will produce a smaller Rf value if the solvent and paper are both changed?

A: Increasing the polarity of solvent will result in increase in Rf value as the compound in even for…

Q: A piece of filter paper is placed in the TLC developing chamber to: Choose one: provide a lining…

A: The correct option is (c) It is because filter paper in used in developing chamber  as it absorbs…

Q: improve

A: We know that resolution is a measure of how well two Elution peak can be differentiated in…

Q: 1.what data must be available when HPLC method is passed on 2.why eluents should be degassed

A: 1.data needed for HPLC: HPLC is High performance liquid chromatography. Chromatography:  It is a…

Q: Question 5 Which part of the HPLC contains majority of the solvent? O fraction collector O reservoir…

A: The beginning of the HPLC system occurs with the solvent reservoir which contains the solvent , and…

Q: What happens if the spots are made too small when preparing a TLC plate for development?

A:

Q: The following conditions may not cause tailing EXCEPT a. Thick filter paper as the stationary phase…

A: Tailing in thin layer chromatography :  Due to the using high concentration of solute or other…

Q: How to get maximum % recovery from recrystallization and crude weight

A:

Q: How column is developed and solvent selection is done in HPLC?

A: HPLC stands for High-pressure liquid chromatography or high-performance liquid chromatography.HPLC…

Q: Rf of spot A in the TLC plate below is: Solvent front A 10 cm OB 8 cm 6 cm 2 cm Origin Select one: O…

A: The Rf value of a component depends on the distance travelled by the component and the distance…

Q: Identification of spots on the TLC plate is done by all of the following EXCEPT O Spraying with…

A: TLC is thin layer chromatography used to know the components in mixture and generally used to check…

Q: What is the line at the top of a TLC called? Select one: The origin O The end line O The solvent…

A: TLC is commonly known as thin - layer chromatography, in this method non- volatile mixture are…

Q: Draw a skitch for GC and HPLC instruments showing all parts

A: GC and HPLC instrumentation can be given as follows

Q: How do you weigh hydgroscopic samples?

A: Hydgroscopic samples contain moisture. Therefore it is measured in digital analytical balance.

Q: When would using Thin Layer Chromotography (TLC) NOT be useful in conducting an oxidation reaction?…

A: Although it is a very simple and convenient technique, one of its limitations is that it cannot tell…