Bio-123 Molecular Biology Part 1 Post/ Part 2 Pre Lab The following is a restriction map for the DNA of YIPS, a plasmid that is 5,541 base pairs (bp). The numbers indicate the location (in terms of the base pairs) of the restriction sites for each enzyme, Use the maps to answer the following questions Hindll 32 EcoR1 1/5541 Pvul 4916 Eagl 942 Apal 2035 Pvull 3247 Smal 2540 5. How many fragments would be produced if this plasmid was digested with both EcoRI and Eagl? a. What would be the size of each fragment? 6. How many fragments would be produced if this plasmid was digested with three enzymes: Hind II, Apal & Pvu lI? a. What would be the size of each fragment? 7. How many fragments would be produced if this plasmid was digested with four enzymes: Hindlll, Apol Pvul, & Pvull? a. What would be the size of each fragment? Molecular Biology Part 1-2 Post Lab V1 Short.Docx Page 4 Revised 12/2/2019

Bio-123 Molecular Biology Part 1 Post/ Part 2 Pre Lab The following is a restriction map for the DNA of YIPS, a plasmid that is 5,541 base pairs (bp). The numbers indicate the location (in terms of the base pairs) of the restriction sites for each enzyme, Use the maps to answer the following questions Hindll 32 EcoR1 1/5541 Pvul 4916 Eagl 942 Apal 2035 Pvull 3247 Smal 2540 5. How many fragments would be produced if this plasmid was digested with both EcoRI and Eagl? a. What would be the size of each fragment? 6. How many fragments would be produced if this plasmid was digested with three enzymes: Hind II, Apal & Pvu lI? a. What would be the size of each fragment? 7. How many fragments would be produced if this plasmid was digested with four enzymes: Hindlll, Apol Pvul, & Pvull? a. What would be the size of each fragment? Molecular Biology Part 1-2 Post Lab V1 Short.Docx Page 4 Revised 12/2/2019

Name: number 6 and 6a Bio-123 Molecular Biology Part 1 Post/ Part 2 Pre LabT
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Description: number 6 and 6a Bio-123 Molecular Biology Part 1 Post/ Part 2 Pre LabThe following is a restriction map for the DNA of YIPS, a plasmid that is 5,541 base pairs (bp).The numbers indicate the location (in terms of the base pairs) of the restriction sit

Human Heredity: Principles and Issues (MindTap Course List)

11th Edition

ISBN:9781305251052

Author:Michael Cummings

Publisher:Michael Cummings

Chapter13: An Introduction To Genetic Technology

Section: Chapter Questions

Problem 23QP

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HersheyChase Experiments The graph shown in FIGURE 8.5 is reproduced from an original 1952 publication by Hershey and Chase. Bacteriophage were labeled with radioactive tracers and allowed 10 infect bacteria. The virusbacteria mixtures were then whirled in a blender to dislodge any viral components attached to the exterior of the bacteria. Afterward, radioactivity from the tracers was measured. FIGURE 8.5 Detail of Alfred Hershey and Martha Chases 1952 publication describing their experiments with bacteriophage. Infected bacteria refers to the percentage of bacteria that survived the blender. After 4 minutes in the blender, what percentage of each isotope was extracellular?
HersheyChase Experiments The graph shown in FIGURE 8.5 is reproduced from an original 1952 publication by Hershey and Chase. Bacteriophage were labeled with radioactive tracers and allowed 10 infect bacteria. The virusbacteria mixtures were then whirled in a blender to dislodge any viral components attached to the exterior of the bacteria. Afterward, radioactivity from the tracers was measured. FIGURE 8.5 Detail of Alfred Hershey and Martha Chases 1952 publication describing their experiments with bacteriophage. Infected bacteria refers to the percentage of bacteria that survived the blender. The extracellular concentration of which isotope increased the most with blending?
HersheyChase Experiments The graph shown in FIGURE 8.5 is reproduced from an original 1952 publication by Hershey and Chase. Bacteriophage were labeled with radioactive tracers and allowed 10 infect bacteria. The virusbacteria mixtures were then whirled in a blender to dislodge any viral components attached to the exterior of the bacteria. Afterward, radioactivity from the tracers was measured. FIGURE 8.5 Detail of Alfred Hershey and Martha Chases 1952 publication describing their experiments with bacteriophage. Infected bacteria refers to the percentage of bacteria that survived the blender. Before blending what percentage of each isotope. 35S and 32P, was extracellular (outside the bacteria)?
HersheyChase Experiments The graph shown in FIGURE 8.5 is reproduced from an original 1952 publication by Hershey and Chase. Bacteriophage were labeled with radioactive tracers and allowed 10 infect bacteria. The virusbacteria mixtures were then whirled in a blender to dislodge any viral components attached to the exterior of the bacteria. Afterward, radioactivity from the tracers was measured. FIGURE 8.5 Detail of Alfred Hershey and Martha Chases 1952 publication describing their experiments with bacteriophage. Infected bacteria refers to the percentage of bacteria that survived the blender. Do these results imply that viruses inject DNA or protein into bacteria? Why or why not?
INSTRUCTION: Given the DNA sequence below, provide the answers to the following items. a. complimentary DNA strand 1.) G A A A T G A C C A G A T T T A T G G C C T G A 2). A T G C G A C C T T A A G T C A A T T G C G A C b. mRNA 1.) G A A A T G A C C A G A T T T A T G G C C T G A 2). A T G C G A C C T T A A G T C A A T T G C G A C c. protein synthesized 1.) G A A A T G A C C A G A T T T A T G G C C T G A 2). A T G C G A C C T T A A G T C A A T T G C G A C
During proofreading, which of the following enzymes reads the DNA? primase topoisomerase DNA pol helicase
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5'-[seq]-3' The diagram shows the results of gel electrophoresis for Sanger sequencing. The wells are represented by open boxes and the DNA bands are represented by black boxes. The wells are labeled to show which dideoxy reaction was loaded into each. Write the sequence of the original template strand used for this sequencing reaction, with the 5’ end on the left and the 3’ end on the right.
Preparing plasmid DNA (double stranded, circular) for Sanger sequencing involves annealing a complementary, single-stranded oligonucleotide DNA primer to one strand of the plasmid template. This is routinely accomplished by heating the plasmid DNA and primer to 90°C and then slowly bringing the temperature down to 25°C. Why does this protocol work? What enzyme is used and what other components are required in the sequencing reaction? How does the Sanger method determine the sequence?
Plasmid Mapping (what is it? why is it important?).
expalin Yeast centromeric DNA sequences