Biochemistry: Site-directed mutagenesis, in which individual amino acid residues are replaced with others, is a powerful method to study enzyme mechanisms. In experiments with particular enzyme, various lysine residues were replaced with aspartate, yielding the results summarized in the table below: Enzyme Form: Enzyme Activity (U/mg) Native enzyme: 1,000 U/mg Recombinant Lys 21 to Asp 21: 970 U/mg Recombinant Lys 86 to Asp 86: 100 U/mg Recombinant Lys 101 to Asp 101: 970 U/mg a. What might be inferred about the role of Lys 21, 86, and 101 in the catalytic mechanism of this enzyme? b. Discuss where within the enzyme one might find Lys 21 and 101. Are these residues likely to be evolutionary conserved in this enzyme? Explain c. Is Lys position 86 likely to be evolutionary conserved? Explain
Biochemistry:
Site-directed mutagenesis, in which individual amino acid residues are replaced with others, is a powerful method to study enzyme mechanisms. In experiments with particular enzyme, various lysine residues were replaced with aspartate, yielding the results summarized in the table below:
Enzyme Form: Enzyme Activity (U/mg)
Native enzyme: 1,000 U/mg
Recombinant Lys 21 to Asp 21: 970 U/mg
Recombinant Lys 86 to Asp 86: 100 U/mg
Recombinant Lys 101 to Asp 101: 970 U/mg
a. What might be inferred about the role of Lys 21, 86, and 101 in the catalytic mechanism of this enzyme?
b. Discuss where within the enzyme one might find Lys 21 and 101. Are these residues likely to be evolutionary conserved in this enzyme? Explain
c. Is Lys position 86 likely to be evolutionary conserved? Explain
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