Biochemistry techniques: a) Name and briefly describe the ELISA assay with greater sensitivity for detection of the viral infection. Focus on antigens and antibodies. Justify your reasoning. B) Consider a mixture of four proteins with various molecular weights. A histone molecule weighs 15 kDa, a p53 molecule weighs 53 kDa, an actin molecule weighs 42 kDa, and an IgG molecule weighs 150 kDa. Arrange the molecules in order of their elution from a gel filtration column. Elutes first (A) to elute last (D) arrangement below: A) B) C) D) Explain your reasoning . Instructor C) . What characteristics determine the position of a protein at the end of isoelectric focusing of 2D SDS- PAGE?
Biochemistry techniques: a) Name and briefly describe the ELISA assay with greater sensitivity for detection of the viral infection. Focus on antigens and antibodies. Justify your reasoning. B) Consider a mixture of four proteins with various molecular weights. A histone molecule weighs 15 kDa, a p53 molecule weighs 53 kDa, an actin molecule weighs 42 kDa, and an IgG molecule weighs 150 kDa. Arrange the molecules in order of their elution from a gel filtration column. Elutes first (A) to elute last (D) arrangement below: A) B) C) D) Explain your reasoning . Instructor C) . What characteristics determine the position of a protein at the end of isoelectric focusing of 2D SDS- PAGE?
Human Anatomy & Physiology (11th Edition)
11th Edition
ISBN:9780134580999
Author:Elaine N. Marieb, Katja N. Hoehn
Publisher:Elaine N. Marieb, Katja N. Hoehn
Chapter1: The Human Body: An Orientation
Section: Chapter Questions
Problem 1RQ: The correct sequence of levels forming the structural hierarchy is A. (a) organ, organ system,...
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Transcribed Image Text:Biochemistry techniques:
a) Name and briefly describe the ELISA assay with greater sensitivity for detection of the viral infection.
Focus on antigens and antibodies. Justify your reasoning.
B) Consider a mixture of four proteins with various molecular weights. A histone molecule weighs 15
kDa, a p53 molecule weighs 53 kDa, an actin molecule weighs 42 kDa, and an IgG molecule weighs 150
kDa, Arrange the molecules in order of their elution from a gel filtration column. Elutes first (A) to elute
last (D) arrangement below:
A)
B)
C)
D)
Explain your reasoning . Instructor
C). What characteristics determine the position of a protein at the end of isoelectric focusing of 2D SDS-
PAGE?
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