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- Why mixture of solvents such as ethyl acetate with 5% acetic acid is used as a solvent/mobile phase for developing TLC? Please explain in detail.what is more polar, pyrrole or benzaldehyde? I need to know for a TLC test/ which one should be further up?could products be isolated for further analysis suing TLC (thin-layer chromotography) as a seperation technique? explain
- An unknown mixture was analyzed with TLC on a silica gel plate. Development gave two spots with Rf values of 1.0 and 0.0 respectively.a) Can you say for sure how many compounds are present in the mixture? If so, how many are there? Explain. b) If not, what additional TLC experiment(s) might be performed to determine how many components are present? Explain.Consider stereoisomers. What type of isomers would you expect would and what types could not be separted by chromatography techniques. also include specifically thin layer chromotography and why, explainOn the blank TLC provided to the right, please draw where you would expect to see the spots for B-carotene (lane 1), compound A (lane 2) and compound B (lane 3). This is SiO2 plate and the appropriate developing solvent is used.
- SEE PHOTO: Effectiveness of separation. Based on the TLC analysis, did the isolated benzoic acidappear pure relative to the starting mixture? Did the isolated naphthalene look pure?How could the purity of the isolated materials be improved?Explain why it is needed to filter the solvent before introducing it to the HPLC systemWhat is the best solvent system for TLC that would elute this compound? And why is that solvent mixture is appropriate?
- What is the order in which the following compounds would he eluted from an HPLC column containing a reversed -phase packing?(a) benzene, diethyl ether, n-hexane(b) acetone, dichloroethane, acetamideSEE PHOTO: Effectiveness of separation. Based on the TLC analysis, did the isolated benzoic acidappear pure relative to the starting mixture? Did the isolated naphthalene look pure?How could the purity of the isolated materials be improved? In the picture: Aq- is Benzoic acid O- is Naphthalene both- is both Benzoic acid and NaphthaleneWhat is the structure and IUPAC name of atrazine? The herbicide simazine is more polar than atrazine. In reverse phase chromatography of a solution containing a mixture of the two substances, which would elute first? Explain why this is in terms of partition. The instrument used in this practical exercise has a UV spectrophotometer as a detector. Give two other types of chromatographic detectors that might be used.