Calculate the growth rate and generation time of a culture that increases in the exponential phase from 5x102 to1x108 cells in 12 hours

With liquid media such as broth, is it possible to determine if the culture is pure using the naked eye only? no yes sometimes

During the heat shock step, a student accidentally heated the cells at 42°C for 5 minutes. Only a few colonies were found on the agar plates at the end of the experiment. Explain the results.

Develop a spreadsheet to predict as functions of time with concentration, volimme and permeate flows for a batch ultrafiltration. Assume that the flux depends on concentration as follows: J(L/M²/H) = A*EXP(-bC) where C is the retentate concentration in g/l. Assume that the recirculation rate is kept high enough that shear rate dependence is eliminated. Do a sample run with C.= 0.5 g/l, Cfinal = 50 g/l, A = 50, and b = 0.02. Assume a filtration area of 19.4M² and an initial volume of 3255 L. Assume σ (protein) = 1.0 or σ (protein) = 0.95 or σ (protein) = 0.90.

Describe how a Triple Sugar Iron agar tube would appear if the following situation was true. Please note that all items in the following list are occurring in the same tube of agar. 1. Positive for glucose fermentation 2. Negative for lactose and sucrose fermentation 3. Positive for H2S production 4. Negative for gas production

Based on the growth curve, which ONE of the following best describes what would happen if sample of culture in the presence of drug B is inoculated onto drug-free growth medium?   Select one: A.   Growth present, but cell count increased by at least 99.9% of the original culture   B. No growth after 24 hrs C. Growth at 24 hrs D. Not possible to predict because the culture could either be alive or dead E. Growth present, but cell count reduced by at least 99.9% of the original culture

You are growing a culture of S. aureus. You know that 9 generations have occurred after 6 hours. What is the generation time in minutes? if i started a culture with 1000 cells, after 8 generations, how many cells will i have?

In disc diffusion studies, where will the lowest concentration of the tested chemical be in the agar plate? in the agar directly below the disc O in the agar halfway between the disc and the petri dish wall O in the agar furthest away from the disc the cehmical is equally distributed throughout the agar

Which of the following terms are used to describe the appearance of the edge or margin of a colony growing on an agar surface? Check all that apply turbid entire undulate convex lobate umbonate

The following is result from a standard plate count. What is the cell density of the undiluted culture? Please follow all SPC rules. (TNTC: too numerous to count)