Calculate the transformation efficiency of an experiment conducted using 10ul of 0.005 ug/ul plasmid, you plated 100ul out of a total volume of 500ul and the count of transformed colonies was 186.

17 A nutrient agar plate labeled 10-5 mL produced 154 colonies after incubation. a. What was the cell density in the original sample? ET b. What combination(s) of volumes and dilution factors could have been used to inoculate this plate? A nutrient agar plate labeled 10-7 mL produced 62 colonies after incubation. a. What was the cell density in the original sample? b. What combination(s) of volumes and dilution factors could have been used to inoculate this plate? The original cell density in a sample is 2.79 x 100 CFU/mL. Which sample volume should yielda countable mL.) plate? (Express your answer as 10 20 The original cell density in a sample is 5.1 x 10° CFU/mL. Which sample volume should yield a countable plate? (Express your answer as 10 mL.) 21 A sample has a density of 1.37 x 105 CFU/mL. a. What sample volume should yield a countable plate? b. Which two dilution tubes could be used to produce this sample volume? How? 22 A sample has a density of 7.9 x 10° CFUlmL. a. What sample…

step by step calculations please

Assume you have successfully cloned a small (200 bp) fragment of DNA into the polylinker region of a pUC18 cloning vector. Describe the appearance of transformed colonies you would expect to see on each of the following plates: plain media, media containing ampicillin, media containing tetracycline, media containing ampicillin and X-Gal.

The modifiedplasmid is reintroduced back into Rhizobium(step 4) and the genetically transformed bacteria are then selected based on the amp and lacZgenes present within the plasmid. The plasmid may or may not integrate the BBW resistancegene. The treated bacteria may or may not take up the modified plasmid.   a) Complete the table below with a yes or no in each space stating whether you would expect these bacteria to grow or not. Type of treated bacteria culture plate(no amp) Culture plate treated with ampicillin   Plasmid not taken up     Plasmid taken up (WithoutBBW resistancegene)     Plasmid taken up (WithBBW resistancegene)     B) Outline how the genetically transformed bacteria containing the BBW resistance gene can beselected based on the amp and lacZgenes present within the plasmid.

EcoRI 1.1kbp 1.5 kbp Hindll Hindll 0.15 kbp amp 0.25 kbp EcoRI If you were to digest this plasmid with HindllI, how many fragments would be visible using gel electrophoresis? O 1 O 4

Can this standard curve be used for E. coli cells (why/why not?)