Can you please discuss the principles of using Motility Test Medium for the detection of bacterial motility. Focus on the components of the medium and the purpose of each component. Note: You may refer to different culture media manuals like Difco, BBL, etc.
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Can you please discuss the principles of using Motility Test Medium for the detection of bacterial motility. Focus on the components of the medium and the purpose of each component. Note: You may refer to different culture media manuals like Difco, BBL, etc.
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- discuss why a mixed culture cannot be used to inoculate a differential media such as the tripple sugar iron agar test.Why can EMB agar be used to detect the presence of E. coli in particular? Explain the principle of detection of the organism using EMB agarAre the large numbers of microorganisms found in the mouth cause for concern? Explain. How can you determine whether a culture or subculture is pure? What kinds of clinical speciments may yield a mixed flora in bacterial cultures? Why was a blood agar, rather than a nutrient agar, plate used for the culture from your mouth?
- What concentration of agar is used in a semisolid medium for motility determination? How does that compare to a typical solid medium ? Explain.What concentration of agar is used in a semisolid medium for motility determination?What is it called when you stab the nutrient agar during the inoculation step of isolating a microorganism?
- Given the illustration and values below, determine the concentration of the original sample. Report results in CFU/mL or colony-forming unit/milliliter. Note that in order to observe the accuracy of results, culture plates with countable colonies between 25-250 CFU are considered in standard bacterial plate count.When using Endo agar as the selective and differential media for the confirmed test, how would you know whether the test is positive for the presence of coli?The students of a Microbiology class were tasked to transfer or subculture a pure culture of Escherichia coli bacterium in five 7 mL nutrient broth and five petri dishes of nutrient agar with 20 mL capacity each. Based on the instruction bottles for nutrient broth and nutrient agar, preparation of the culture media is as follows. Nutrient broth: 8 g/liter Nutrient agar: 28 g/liter Answer the following: a. What is the weight in grams of nutrient broth? b. What is the weight in grams of nutrient agar? c. What is the distilled water in mL for nutrient broth? d. What is the distilled water in mL for nutrient agar?
- What is the purpose of the pour plate technique? If a pure culture is used to inoculate the plate, why are some colonies bigger than others?Your instructor asks you to isolate and identify the organisms in an unknown culture. You find that the culture contains two gramnegative bacilli that produce swarming colonies. What biochemical test would you use to identify the bacilli? Justify your answer.In the preparation of a bacterial smear, why is there a need to fix the bacteria to the slide? Aside from passing the slide over a flame, what are the other ways of fixing the bacteria to the slide?