Ch 3. Two mobile phases were evaluated to study the behavior of five dyes. The following data from each chromatogram were collected: CPLOWET oite Owaiva Mobile Phase 1: Acetone/Water Mobile Phase 2: Ethanol/Water Distance RF Distance RF traveled (cm) noina traveled (cm) Solvent front 4.7 4.9 Green dye Red dye Yellow dye Blue dye Purple dye ,१४on .76cm Tocm .53em .29cm 2.9 .62 cm 4.8 1.3 3.7 4.0 0.8 2.9 .62(m 2.6 3.1 1.4 a) Calculate the RF values for each dye for both chromatographic systems. Record these values in the table above. a) Calculate the RF values for each dye for both chromatographic systems. Record these values in the table above. b) Explain your reasons for choosing which solvent would be most effective in separating the green from the blue dye. olduloz esal c) Which mobile phase system would be most effective for separating all 5 dyes? Explain.

Question

Explain your reasons for choosing which solvent would be most effective in separating the green from blue dye.

Ch
3. Two mobile phases were evaluated to study the behavior of five dyes. The following
data from each chromatogram were collected:
CPLOWET
oite
Owaiva
Mobile Phase 1: Acetone/Water
Mobile Phase 2: Ethanol/Water
Distance
RF
Distance
RF
traveled (cm)
noina
traveled (cm)
Solvent front
4.7
4.9
Green dye
Red dye
Yellow dye
Blue dye
Purple dye
,१४on
.76cm
Tocm
.53em
.29cm
2.9
.62 cm
4.8
1.3
3.7
4.0
0.8
2.9
.62(m
2.6
3.1
1.4
a) Calculate the RF values for each dye for both chromatographic systems. Record these
values in the table above.
View transcribed image text
Expand
a) Calculate the RF values for each dye for both chromatographic systems. Record these
values in the table above.
b) Explain your reasons for choosing which solvent would be most effective in
separating the green from the blue dye.
olduloz esal
c) Which mobile phase system would be most effective for separating all 5 dyes? Explain.
View transcribed image text
Expand

Expert Answer

Want to see the step-by-step answer?

Check out a sample Q&A here.

Want to see this answer and more?

Experts are waiting 24/7 to provide step-by-step solutions in as fast as 30 minutes!*

*Response times may vary by subject and question complexity. Median response time is 34 minutes for paid subscribers and may be longer for promotional offers.
Tagged in
Science
Chemistry

Quantitative analysis

Related Chemistry Q&A

Find answers to questions asked by students like you.

Q: The reduction potential for the following non-standard half cell at 298K is volts. Cl2 (4.92x10*atm)...

A: The standard reduction potential for the given half-cell reaction is:

Q: A solution of permanganate is standardized by titration with oxalic acid (H2 C204). It required 58.5...

A: Number of moles of oxalic acid can be calculated as follows:

Q: What is the temperature of 0.210 moles of an ideal gas whose pressure is 42.0 atm and volume is 0.12...

A: Click to see the answer

Q: At a certain temperature, the equilibrium constant, Kc, for the reaction is 4.00. An equilibrium mix...

A: Given information:  Kc = 4.00  V = 1.00 L 4.60 moles of NH3(g) 3.00 moles of Cl2(g) 5.90 moles of N...

Q: In an ammonia reaction if the temperature is increased which direction will equilibrium shift? If th...

A:                

Q: Prepare 150 mL of a 3% agarose gel solution. Specify the following quantities necessary for this pre...

A: Formation of Agarose gel is a technique used to separate various macromolecules.

Q: A solution contains the following ions: Na+ (15 mEq/L), K+ (10. mEq/L), and Ca2+ (4 mEq/L). If Cl− i...

A: Since the sum of equivalents of cations in any solution should be equal to the sum of equivalents of...

Q: Maybelline Cousteau's backup oxygen tank reads 900 mmHg while on her boat, where the temperature is ...

A: The pressure in the backup tank at -183 C is to be calculated.

Q: will not be available until you sign in again. View earning Ch 7 HW Normandale Community College - C...

A: Since you have posted multiple question, we are entitled to answer the first one only.

Q: Ecell (positive or negative) %3D Keq = (> 1 or < 1) %3D Cell Potential and Concentration When an ele...

A:                  

Q: Describe the effect of pH on the solubility of Fe(OH)3

A: A reaction is said to be in equilibrium if the rate of the forward reaction is equal to the rate of ...

Q: Consider the following balanced chemical reaction:   H2SO4 (aq) + 2 KOH (aq) ------------&gt; K2SO4 ...

A: We first consider a balanced chemical equation : H2SO4+2KOH------------&gt;K2SO4+2H2O This shows tha...

Q: What is a polymer? a. any organic compound made by combining smaller molecules b. any synthetic orga...

A: A polymer is defined as a large molecule, or macro-molecule, composed of many repeated sub-units. It...

Q: How many structural isomers are there of C4H10? Select one: A. 4 B. 6 C. 8 D. 2

A: Please find below the all possible structural isomers of C4H10 

Q: The temperature of a gas is increased from 1250C to 1820C inside of a rigid container. The original ...

A: Given: T1= 1250 C T2= 1820 C P1= 1.22 atm P2= ?  

Q: When the Ag* concentration is 8.50×10-4 M, the observed cell potential at 298K for an electrochemica...

A: Click to see the answer

Q: What is the IUPAC name for the following alkane? CH3 CH-CH2-CH3 CH CH;

A: IUPAC name:  

Q: A sample of N2(g) occupies a volume of 42.5 mL under the existing barometric pressure. Increasing th...

A: As per Boyle's law volume of given amount of an ideal gas is inversely proportional to the pressure ...

Q: The average distance between the earth and moon is 3.85 × 105 km. If you shined a laser pointer towa...

A: Answer :- The speed of the light in vacuum = 3x108 m/s. Speed= distance/time. distance given =3.85 x...

Q: In order for a gas-filled balloon to rise in air, the density of the gas in the balloon must be less...

A: Click to see the answer

Q: The urms of H2 molecules at 273K is 1.84×103m/s. At what temperature is urms for H2 1.8 times greate...

A: Since the urms of any molecule is given by  Hence we can say  urms T1 / urms T2 = (T1 / T2)1/2 sinc...

Q: What is the volume of 1.32 moles of an ideal gas whose pressure is 0.45 atm and temperature is 311 K...

A: Using the ideal gas equation: PV = nRT

Q: I wanted to know why the Suzuki reaction can take place in aqueous solution whereas a Grignard react...

A: Click to see the answer

Q: NaOH(s) trial (A) NHẠNO3 (s) trial (B) Part 2: Enthalpy of dissolution a. Mass of substance dissolve...

A: Since you have posted a question with multiple sub-parts, we will solve first three sub-parts for yo...

Q: Liquid hexane  CH3CH24CH3  reacts with gaseous oxygen gas O2 to produce gaseous carbon dioxide CO2...

A: The balanced reaction taking place is (C6H14 = hexane) C6H14 + 9.5 O2 ----&gt; 6 CO2 + 7 H2O Hence f...

Q: At what temperature does C₂H₂Cl₂ have a density of 0.812 g/L at 1.17 atm?   Please include calculati...

A: The ideal gas equation is given below. PV = nRT PV = (m / M) RT (PM) / (RT) = m / v (PM) / (RT) = d…...

Q: A 0.15 M solution of a weak acid (HA) has a pH of 2.35. What is the Ka for the acid? (Note: you shou...

A: Given: pH of weak acid= 2.35 Molarity of weak acid= 0.15 M  

Q: How many grams of dry NH4C1 need to be added to 2.50 L of a 0.800 M solution of ammonia, NH3 , to pr...

A: NH3 + NH4Cl forms a basic buffer. For a basic buffer:  pOH = pKb + log c2/c1 pH + pOH = 14

Q: I need help solving this

A: In the given chemical equation, NH3 (g), N2 (g), and H2 (g) are gaseous state. On decomposition of t...

Q: Water freezes spontaneously at -5°C and 1atm. Describe how a spontaneous process can result in a dec...

A: Entropy usually refers to the randomness of a system. A reaction is said to be spontaneous if it occ...

Q: Which is the best acid to use in the preparation of a buffer with pH =3.3? A. HNO3 B. HOI (Ka = 2.0 ...

A: EXPLANATION Step 1 Buffer solutions are prepared by weak acids and their salts. If we want to prepar...

Q: What is the pH of a buffer solution of 0.0025 M CH 3CO 2H and 0.00125 M CH 3CO 2Na? ( K a = 1.8 × 10...

A: Henderson-Hasselbalch equation Henderson-Hasselbalch equation explains the relationship between pH o...

Q: A blood concentration of 0.065M ethyl alcohol (EtOH) is sufficient to induce a coma. At this concent...

A: Since concentration = moles of solute / volume of solution in L Solute here is ethanol And volume of...

Q: A balloon is filled to a volume of 1.50 L with 3.00 moles of gas at 25 degrees celcius. With pressur...

A: According to ideal gas law, PV = nRT where P = pressure of gas V = volume n = moles R = gas constant...

Q: Which two elements have 3 unpaired 3d electrons in the ground state?

A: As per "Aufbau principle", the orbital that contains less energy gets filled first as compared to th...

Q: If PbI2(s) is dissolved in 1.0 M NaI(aq), is the maximum possible concentration of Pb2+(aq) in the s...

A: The mathematical expression for the solubility products is shown in equation (1) where [Pb2+] and [I...

Q: Determine the equilibrium constant (Kp) at 25.0°C  (298.15K) for the reaction CO(g) + H2O(g)  &lt;--...

A: The equation connecting free energy change and equilibrium constant is given below:

Q: Draw the organic products of the following two reactions of the L-threose

A: Copper ion is an oxidizing agent.  It oxidizes aldehyde part of L-threose to carboxylic acid. 

Q: Calculate G° for each reaction at 298K using G°f values.(a) 2 Mg(s) + O2(g)--&gt; 2 MgO(s)(b) MnO2(s...

A: The difference between the free energies of product and the reactants is known as change in Gibb’s f...

Q: Calculate AGo for the following reaction: 4CO (g) + 2NO2(g)→4CO2 (g) +N2 (g). Use the following reac...

A: GIVEN:  

Q: 3. Two mobile phases were evaluated to study the behavior of five dyes. The following data from each...

A: (a) Retention factor (Rf) is the ratio of the distance moved by the solute (dye) to the distance mov...

Q: Which of the following would be MOST soluble in water? Select one: A. chloropropane B. ethanol C....

A: As we know, Water is a polar molecule . And , we know polar dissolve polar and non- polar dissolve ...

Q: The tertiary structure of a protein is stabilized by several types of interactions between side chai...

A: The structure of the protein is largely dependent on the interactions of the side chain bonds.  It i...

Q: Consider the following reaction:  Ni(s) + 4CO(g) &lt;--&gt;Ni(CO)4(g) At 1397o C, the standard free ...

A: Since the relationship between free energy change and standard free energy change is given by  ΔG = ...

Q: If the flask were not wiped completely dry, how would the derived mass of the gas affect the molar m...

A: If the flask were not wiped completely dry, then some water will be stuck onto the flask. This water...

Q: A 1.0 M solution of aluminum nitrate is placed in a beaker with a strip of aluminum metal. A 1.0 M s...

A: Click to see the answer

Q: Quinapril (trade name Accupril) is used to treat high blood pressure and congestive heart failure. O...

A: Click to see the answer

Q: What reactions take place at the cathode and the anode when each of the following is electrolyzed? A...

A: (a) The electrolysis of solution of ZnCl2 is represented by the reactions taking place at the cathod...

Q: 1. How many grams of Cu would have to react to yield 89.5 grams of 2 p silver? Cu + 2 AGNO3 → Cu(NO3...

A: Click to see the answer

Q: Calculate the temperature of a gas when it is expanded to 5.25L. The gas originally occupies 3.45L o...

A: Given, V1 = 3.45L T1 = 282K V2 = 5.25L T2 = ?

Transcribed Image Text

Ch 3. Two mobile phases were evaluated to study the behavior of five dyes. The following data from each chromatogram were collected: CPLOWET oite Owaiva Mobile Phase 1: Acetone/Water Mobile Phase 2: Ethanol/Water Distance RF Distance RF traveled (cm) noina traveled (cm) Solvent front 4.7 4.9 Green dye Red dye Yellow dye Blue dye Purple dye ,१४on .76cm Tocm .53em .29cm 2.9 .62 cm 4.8 1.3 3.7 4.0 0.8 2.9 .62(m 2.6 3.1 1.4 a) Calculate the RF values for each dye for both chromatographic systems. Record these values in the table above.

a) Calculate the RF values for each dye for both chromatographic systems. Record these values in the table above. b) Explain your reasons for choosing which solvent would be most effective in separating the green from the blue dye. olduloz esal c) Which mobile phase system would be most effective for separating all 5 dyes? Explain.