Chart is Given for you: Below is a chart of values for actual enzymes. Enzyme                Km (M)                       kcat (1/s)Chymotrypsin       1.5 × 10^−2                   0.14Pepsin                   3.0 × 10^−4                   0.5Tyrosyl-tRNA synthetase 9.0 × 10^−4        7.6Ribonuclease        7.9 × 10^−3               7.9 × 10^2Carbonic anhydrase 2.6 × 10^−2             4.0 × 10^5Fumarase               5.0 × 10^−6                  8.0 × 10^2 Assume the enzyme concentration is equal across all samples (and is equal to 1). (Answer a and b only)a. Which enzyme will have the highest V0 at very high substrate concentrations? (1 M). Why? b. Which will have the highest V0 at very low substrate concentrations (5.0 × 10^−12). Why?

Biochemistry
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Chapter26: Synthesis And Degradation Of Nucleotides
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Chart is Given for you: Below is a chart of values for actual enzymes.

Enzyme                Km (M)                       kcat (1/s)
Chymotrypsin       1.5 × 10^−2                   0.14
Pepsin                   3.0 × 10^−4                   0.5
Tyrosyl-tRNA synthetase 9.0 × 10^−4        7.6
Ribonuclease        7.9 × 10^−3               7.9 × 10^2
Carbonic anhydrase 2.6 × 10^−2             4.0 × 10^5
Fumarase               5.0 × 10^−6                  8.0 × 10^2


Assume the enzyme concentration is equal across all samples (and is equal to 1).

(Answer a and b only)
a. Which enzyme will have the highest V0 at very high substrate concentrations? (1 M). Why?

b. Which will have the highest V0 at very low substrate concentrations (5.0 × 10^−12). Why?

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