chromatography on Bio-Gel P-300. The column had a length of 37 cm and a 1.5-cm diameter. Eluate fractions of 0.65 mL were collected. The maximum of each peak came at the following fractions: ferritin, 22; transferrin, 32; and ferric citrate, Ferritin (molecular mass 450 000), transferrin (molecular mass 80 000), and ferric citrate were separated by size exclusion 84. (That is, the ferritin peak came at an elution volume of 22 × 0.65 mL = 14.3 mL.) Assuming that ferritin is eluted at the interstitial volume and that ferric citrate is eluted at Vm, find Kay for transferrin. Kav %3D

Fundamentals Of Analytical Chemistry
9th Edition
ISBN:9781285640686
Author:Skoog
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Chapter33: High-performance Liquid Chromatography
Section: Chapter Questions
Problem 33.15QAP
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Ferritin (molecular mass 450 000), transferrin (molecular mass 80 000), and ferric citrate were separated by size exclusion
chromatography on Bio-Gel P-300. The column had a length of 37 cm and a 1.5-cm diameter. Eluate fractions of 0.65 mL
were collected. The maximum of each peak came at the following fractions: ferritin, 22; transferrin, 32; and ferric citrate,
84. (That is, the ferritin peak came at an elution volume of 22 x 0.65 mL = 14.3 mL.) Assuming that ferritin is eluted at
the interstitial volume and that ferric citrate is eluted at VM, find Kay for transferrin.
Kay =
Transcribed Image Text:Ferritin (molecular mass 450 000), transferrin (molecular mass 80 000), and ferric citrate were separated by size exclusion chromatography on Bio-Gel P-300. The column had a length of 37 cm and a 1.5-cm diameter. Eluate fractions of 0.65 mL were collected. The maximum of each peak came at the following fractions: ferritin, 22; transferrin, 32; and ferric citrate, 84. (That is, the ferritin peak came at an elution volume of 22 x 0.65 mL = 14.3 mL.) Assuming that ferritin is eluted at the interstitial volume and that ferric citrate is eluted at VM, find Kay for transferrin. Kay =
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