Consider stereoisomers. What type of isomers would you expect would and what types could not be separted by chromatography techniques. also include specifically thin layer chromotography and why, explain
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Consider stereoisomers. What type of isomers would you expect would and what types could not be separted by chromatography techniques.
also include specifically thin layer chromotography and why, explain
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- What is the structure and IUPAC name of atrazine? The herbicide simazine is more polar than atrazine. In reverse phase chromatography of a solution containing a mixture of the two substances, which would elute first? Explain why this is in terms of partition. The instrument used in this practical exercise has a UV spectrophotometer as a detector. Give two other types of chromatographic detectors that might be used.How might the Rf values of colorless substances be determined? That is, how could the colorless spots be located? (for paper chromatography)Doe hydrophobic silica gel act as non-polar stationary phase in TLC making the process as reverse chromatography? explain
- Silica gel is very polar stationary phase. Therefore, more polar molecule sticks to silica gel on the TLC plate and has higherRfvalue. True FalsePlease Explain clearly Why couldn’t you use acid-base extraction to separate a ketone and an alcohol? why do we use column chromatography instead? Please explain clearly the benefits of both methods and why is one method preferred over the other.Compound A has a lower affinity for the stationary phase thancompound B and can dissolve well in the mobile phase. In theseparation of the mixture of A and B by column chromatography,which compound will be eluted first from the column? Explain
- Describe briefly, but precisely, what you would have done in order to get to this stage. You can assume that you have been given a suitable thin layer chromatography plate.In HPLC, why is purging being done before analysis of samples. Outline the procedure on how purging is performed.Study the chromatograph (below) of a mixture of Compounds A and B, run on the GCs in the teaching labs at CU Boulder. Compound A has the shorter retention time. You suspect that compound B is octane. What can you do to provide supporting evidence for this hypothesis?
- As distillation method is used to seperate a homogenous mixture, which kind of mixtures chromatography can seperate? Give some example.How could you prepare a sample of pure (S)-naproxen from a sample of racemic naproxen using chromatography?Tell me about the Laboratory experiment: Purifying Protein by Column Chromatography Thank you^^