Create a simple outline (yes, you can use bullets and outlines!) starting from plasmid DNA with your clone in it (pick one, anyone), transform competent cells (choose the cells, describe competent cells and transformation process), and how you would go all the way through from lysis to purification of a GST-Tagged protein.

Create a simple outline (yes, you can use bullets and outlines!) starting from plasmid DNA with your clone in it (pick one, anyone), transform competent cells (choose the cells, describe competent cells and transformation process), and how you would go all the way through from lysis to purification of a GST-Tagged protein.

Biology: The Dynamic Science (MindTap Course List)

4th Edition

ISBN:9781305389892

Author:Peter J. Russell, Paul E. Hertz, Beverly McMillan

Publisher:Peter J. Russell, Paul E. Hertz, Beverly McMillan

Chapter17: Bacterial And Viral Genetics

Section: Chapter Questions

Problem 4TYK

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Similar questions

After a polymerase chain reaction (PCR), agarose gel electrophoresis is often used to: a. amplify the DNA. b. convert cDNA into genomic DNA. c. convert cDNA into messenger RNA. d. verify that the desired DNA sequence has been amplified. e. synthesize primer DNA molecules.
1. Write if the statement is true and modify the statement with the correct answer if its false a.White colonies represent those bacteria that do not express the genes for beta-galactosidase because restriction enzymes have cut the gene and ligase have not repaired the break b.Antibiotic resistance genes in bacterial transformation are used as marker genes because of ease of detection of transformants. c. Bacterial transformation simply means the insertion of a recombinant plasmid into a bacterial cell.
1. How may recombinant DNA molecules be introduced into human cells? a. by splicing the needed genes into a mammalian chromosome using restriction enzymes. b. by adding plasmids to the mammalian cells. c. by using engineered viruses as vectors. d. by using a gene gun. 2. If someone is accused of a crime, investigators can collect his or her DNA to compare the DNA of the cells found at the crime scene. To collect human DNA, investigators often swab the inside of person’s cheek. Just a few human cheek cells contain enough material to perform PCR. In a cell, the nucleus and mitochondria contain DNA that is the starting material for PCR. Identify the 4 components needed to start a PCR reaction (equipment not included)
4. What is the name of the process by which bacteria pick up a different organism’s geneticmaterial?5. Genetically, how does the original bacterial DNA (plasmid) differ from the final bacterial DNAmolecule? (Do not say, “It is longer.”)6. Tetracycline is an antibiotic that is prescribed to kill bacterial infections. The transformedbacterial cell that you created has the tetracycline resistant gene in it. If this cell is placed on agrowth medium with tetracycline, will the bacteria grow or die? Explain.
1. How can site-specific recombination be used in recombinant DNA technology? Answer and explain comprehensively.
1. You are having problems getting good transfection efficiency when you put your plasmid in e. coli. You want to freeze down a stock of e. coli that is more than 99% transfected. What modifications can you make to your plasmid and what steps can you take to ensure that 99% of your e. coli are transfected?
1. The extraction of DNA is a common procedure in biotechnology research laboratories. Research the methods of extracting DNA in biotechnology facilities. Describe how the extraction methods used in this lesson compare to those of research laboratories.
1) Follow the steps involved in DNA or gene cloning 2) In what process do bacteria take up the recombinant plasmid DNA 3) What are the advantages or replications of gene cloning?
1. What makes CRISP particularly useful compared to other genetic engineering techniques? A. CRISPR can add DNA to multiple cells at the same time B. CRISPR works better in bacteria C. CRISPR can target one specific area of DNA to cut D. CRISP is capable of transporting DNA through the cell walls of plants 2. MOST viruses are capable of infecting A. many different species B. many cell types in the same species C. only specific cell types that have a particular receptor
9) The diagram to the right represents one technique used in biotechnology. The organic compound used to cut the bacterial DNA so that the human DNA could be inserted a(n)
1. Write if the statement is true and modify the statement with the correct answer if its false a.Blue colonies in the white-blue screening provide sufficient information on the transfer of recombinant DNA in bacterial cells. b.In blue-white screening, it is only the blue colonies that have survived the effect of antibiotics in the media. c.Bacterial transformation simply means the insertion of a recombinant plasmid into a bacterial cell. d.Competent cells are those bacterial cells that can accept the entry or re-entry of plasmids.
4. Explain how and why a researcher might use a DNA probe.